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Cryopreservation of semen from functional sex-reversed genotypic females of the rainbow trout, Oncorhynchus mykiss BABT
Ninhaus-Silveira,Alexandre; Foresti,Fausto; Tabata,Yara Aiko; Rigolino,Marcos Guilherme; Veríssimo-Silveira,Rosicleire.
Cryopreservation of semen from sex-reversed females of rainbow trout aims at rationalizing the production of stocks composed by 100% females. Semen from normal males (M) and two types of genotypic females (R and G), sex-reversed by the oral administration of 17alpha-methyltestosterone, were used. R was obtained by the fertilization of normal eggs with semen of sex-reversed females while G via gynogenetic reproduction. Semen was diluted in an extender solution (glucose 5,4 g, egg yolk 10 ml, dimetil sulfoxide 10 ml, water 80 ml) at 1:3 ratio (semen/extender), stored in straws of 0.5 ml and freezed in a dry container Cryopac CP-65, at -180ºC. Thawing was performed with water at 70ºC for 3 seconds. There were no significant fertilization rate differences...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Oncorhynchus mykiss; Cryopreservation; Sex reversal; Gynogenesis; Semen.
Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132006000100009
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Seminal analysis, cryogenic preservation, and fertility in matrinxã fish, Brycon cephalus (Günther, 1869) BABT
Ninhaus-Silveira,Alexandre; Foresti,Fausto; Veríssimo-Silveira,Rosicleire; Senhorini,José Augusto.
Aiming to improve fish reproduction techniques, the characterization and cryopreservation of semen of Brycon cephalus were performed. The seminal characteristics observed were: an almost transparent, milky semen with a mean volume of 4mL, and sperm concentration of 9.617±1.630 x 10(6) spermatozoa/mm³. Spermatozoa (length = 31.288 ±4.47µm) were of the aquasperm type and displayed a small, round head (length = 1.727 ±0.18 µm; width = 1.752 ±0.17µm) without acrosomal vesicle, nucleus with highly condensed chromatin forming coarse clots and centriolar complex located in the nuclear fossa; a midpiece (length = 2.561±0.44µm), narrowed rearward, with a cytoplasmic canal; and a flagellum (length = 29.521 ±4.37µm). Fertilization tests with thawed semen demonstrated...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Semen characteristics; Cryopreservation; Brycon cephalus; Ultrastructure; Fish.
Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132006000500015
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Estimated frequency of B-chromosomes and population density of Astyanax scabripinnis paranae in a small stream Braz. J. Genet.
Porto-Foresti,Fábio; Oliveira,Claudio; Maistro,Edson Luis; Foresti,Fausto.
A study was made of the frequency of B-chromosomes and the population density of Astyanax scabripinnis paranae (Pisces, Characidae, Tetragonopterinae) from three stretches of the Cascatinha stream (Botucatu, SP). In the first stretch the population was estimated to be about 212 individuals and among 35 karyotyped specimens, 23 carried one macro B-chromosome; in the second stretch the population was estimated to be about 650 individuals and among 20 specimens karyotyped, two possessed one macro B-chromosome; in the third stretch the population was estimated to be about 107 individuals and among 10 specimens karyotyped, one carried one macro B-chromosome. A significant difference was observed in the frequency of macro B-chromosomes in females (57%) and males...
Tipo: Info:eu-repo/semantics/article
Ano: 1997 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-84551997000300004
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Mitochondrial DNA variation in wild populations of Leporinus elongatus from the Paraná River basin Genet. Mol. Biol.
Martins,Cesar; Wasko,Adriane P.; Oliveira,Claudio; Foresti,Fausto.
Leporinus elongatus, a fish species widely distributed throughout the Paraná River basin in South America, is an important fishery resource and a valuable species in aquaculture programs. Despite its great economic importance, several wild populations have been suffering a drastic reduction. The comprehension of its population structure represents an important step for the conservation of these organisms in natural environments, and also for the selection of wild stocks to be used in hatchery programs. In order to understand the genetic-population structure of L. elongatus, D-loop mitochondrial DNA analyses were applied in six wild populations of the species. The results were used to estimate the levels of within and among population genetic variability....
Tipo: Info:eu-repo/semantics/article Palavras-chave: Conservation genetics; D-loop; Genetic variability; Leporinus elongatus; MtDNA.
Ano: 2003 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572003000100006
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A lethal effect associated with polymorphism of the NOR-bearing chromosomes in rainbow trout (Oncorhynchus mykiss) Genet. Mol. Biol.
Porto-Foresti,Fábio; Oliveira,Claudio; Gomes,Eduardo A.; Tabata,Yara A.; Rigolino,Marcos G.; Foresti,Fausto.
Cytogenetic analysis of a rainbow trout stock showed that the nucleolar organizing regions were located subterminally on the long arm of a submetacentric chromosome pair and occurred as a single chromosomal segment (phenotype N1) or as two chromosomal segments separated by a short euchromatic segment (phenotype N2). Cytogenetic analysis also showed that there were N1N1 and N1N2 individuals but no N2N2 individuals. Analysis of the different includedphenotypes incluted that the population was not in Hardy-Weinberg equilibrium (chi² = 19.333; p < 0.01), and that a higher frequency of individuals had the N1N2 phenotype. Experimental crosses involving four males (two N1N1 and two N1N2) and four females (one N1N1 and three N1N2) yielded eight broods. There...
Tipo: Info:eu-repo/semantics/article Palavras-chave: NOR polymorphism; Paracentric inversion; Fish cytogenetics; Rainbow trout.
Ano: 2004 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572004000100009
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Nuclear DNA content of thirty species of Neotropical fishes Genet. Mol. Biol.
Carvalho,Margarida Lima; Oliveira,Claudio; Foresti,Fausto.
The present paper reports nuclear DNA content in 30 Neotropical freshwater fish species and summarizes the data on other Neotropical species presented in the literature. Among Neotropical fishes, the nuclear DNA content ranges from 1.04 ± 0.09 pg/nucleus in Corydoras cf. simulatus (2n = 62) to 248.0 pg/nucleus in Lepidosiren paradoxa (2n = 38). A general analysis of the data obtained in the present study for each species showed that DNA measurements were practically constant at the individual level, while significant differences were observed among individuals of the same population. This observation was valid for all species analyzed and was more evident in those species that presented other karyotypic particularities such as sex chromosomes or...
Tipo: Info:eu-repo/semantics/article
Ano: 1998 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47571998000100009
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Identification and description of distinct B chromosomes in Cyphocharax modestus (Characiformes, Curimatidae) Genet. Mol. Biol.
Santos,Lessandra Viviane De Rosa; Foresti,Fausto; Martins,Cesar; Oliveira,Claudio; Wasko,Adriane Pinto.
Cytogenetic analyses were performed in Cyphocharax modestus, collected at Paranapanema River and Tietê River (São Paulo State, Brazil). A karyotype with 2n = 54 chromosomes was observed in the animals from both Brazilian freshwater river systems. One to four B chromosomes were also detected in individuals from the Paranapanema River, which represents the probable first report of more than a single supernumerary element in a species of the Curimatidae group. C-banding revealed centromeric and telomeric heterochromatin blocks in several chromosomes of the normal karyotype complement of C. modestus. Moreover, while some B chromosomes were characterized by the complete absence of C-bands, others were totally heterochromatic. Although there was a prevalence of...
Tipo: Info:eu-repo/semantics/article Palavras-chave: B chromosomes; C-banding; Curimatidae; Cyphocharax modestus; Supernumerary chromosomes.
Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572008000200019
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Analysis of NOR distribution in cultivated and naturalized stocks of rainbow trout (Oncorhynchus mykiss) Genet. Mol. Biol.
Porto-Foresti,Fábio; Oliveira,Claudio; Tabata,Yara A.; Rigolino,Marcos G.; Foresti,Fausto.
In salmonid species, nucleolar organizing regions (NORs) have frequently been found on a single chromosome pair. However, some other, smaller yet active NORs have also been described. In the present study, NORs were found to be located in different positions on the chromosomes of 12 rainbow trout samples (11 cultivated and one naturalized stocks). Three phenotypes were found: 1) NORs located on two submetacentric chromosomes, in a subterminal position on the short arm (AA); 2) NORs located on two submetacentric chromosomes, in a subterminal position on the long arm (BB); and 3) NORs located on two submetacentric chromosomes, one in a subterminal position on the short arm, and the other in a subterminal position on the long arm (AB). Phenotype AA was...
Tipo: Info:eu-repo/semantics/article Palavras-chave: NOR polymorphism; Fish cytogenetics; Rainbow trout.
Ano: 2002 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572002000400009
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Identities among actin-encoding cDNAs of the Nile tilapia (Oreochromis niloticus) and other eukaryote species revealed by nucleotide and amino acid sequence analyses Genet. Mol. Biol.
Poletto,Andréia B.; Wasko,Adriane P.; Oliveira,Claudio; Azevedo,Alexandre; Carvalho,Robson F.; Silva,Maeli Dal Pai; Foresti,Fausto; Martins,Cesar.
Actin-encoding cDNAs of Nile tilapia (Oreochromis niloticus) were isolated by RT-PCR using total RNA samples of different tissues and further characterized by nucleotide sequencing and in silico amino acid (aa) sequence analysis. Comparisons among the actin gene sequences of O. niloticus and those of other species evidenced that the isolated genes present a high similarity to other fish and other vertebrate actin genes. The highest nucleotide resemblance was observed between O. niloticus and O. mossambicus a-actin and b-actin genes. Analysis of the predicted aa sequences revealed two distinct types of cytoplasmic actins, one cardiac muscle actin type and one skeletal muscle actin type that were expressed in different tissues of Nile tilapia. The...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Actin; Expression pattern; Nile tilapia; Oreochromis niloticus.
Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572008000200031
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Phylogenetic analysis of the order Pleuronectiformes (Teleostei) based on sequences of 12S and 16S mitochondrial genes Genet. Mol. Biol.
Azevedo,Marisa F.C.; Oliveira,Claudio; Pardo,Belén G.; Martínez,Paulino; Foresti,Fausto.
The fish order Pleuronectiformes, composed of 14 families, has two suborders: Psettodoidei (with one family) and Pleuronectoidei (with thirteen families). The relationships among families of Pleuronectoidei and among the genera of their families have extensively been debated and a consensus has not yet been reached. In the present study, partial sequences of the 12S and 16S mitochondrial rRNA genes were obtained from 19 species belonging to the families Achiridae, Bothidae, Cynoglossidae, Paralichthyidae, Pleuronectidae, Scophthalmidae, and Soleidae. Additional sequences of 42 pleuronectiform species were obtained from GenBank. Phylogenetic analyses were conducted by the methods of maximum-parsimony, maximum-likelihood and Bayesian inference. Our results...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Phylogeny; Molecular systematics; Mitochondrial DNA; Fish evolution; Flatfishes.
Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572008000200023
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Nucleotide sequence, genomic organization and chromosome localization of 5S rDNA in two species of Curimatidae (Teleostei, Characiformes) Genet. Mol. Biol.
Santos,Lessandra Viviane de Rosa; Foresti,Fausto; Wasko,Adriane Pinto; Oliveira,Claudio; Martins,Cesar.
The 5S ribosomal DNA (5S rDNA) of higher eukaryotes is organized in repeat units of tandem arrays composed of a 5S rDNA coding region, conserved even among non-related taxa, and a variable non-transcribed spacer sequence (NTS). To contribute to knowledge on the organization and evolution of vertebrate 5S rDNA we used PCR, nucleotide sequencing, Southern blot hybridization and chromosome fluorescence in situ hybridization (FISH) to investigate 5S rDNA tandem repeats in the South American Curimatidae fish Steindachnerina insculpta and Cyphocharax modesta. 5S rDNA repeats of 180 base pairs (bp) from both species were PCR-generated and sequenced evidencing the shortest 5S rDNA monomer so far described in eukaryote species. Southern blotting revealed that both...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Cyphocharax modesta; Steindachnerina insculpta; 5S rDNA; Chromosomes; FISH; Nucleotide sequence.
Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572006000200009
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Editorial Genet. Mol. Biol.
Foresti,Fausto; Galetti Junior,Pedro Manoel.
Tipo: Info:eu-repo/semantics/other
Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572008000200001
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Karyological characterization of four Neotropical fish species of the genus Hisonotus (Teleostei, Loricariidae, Hypoptopomatinae) from distinct Brazilian river basins Genet. Mol. Biol.
Andreata,Artur Antonio; Oliveira,Claudio; Foresti,Fausto.
The karyotypes of four Hisonotus species (two provisionally-named species A and D, H. nigricauda, and H. leucofrenatus) were found to have the same diploid number of 2n = 54 and interstitial silver-staining nucleolus organizer regions (Ag-NORs) located on the long arm of the largest metacentric pair. The C-banding pattern appeared to be species-specific, with one group (H. nigricauda and the unnamed species A and D) being characterized by small amounts of positive C-banded segments and containing a sub-group (species A and D) identified by a large positive C-banded segment on a small metacentric chromosome pair. The second group contained different samples of H. leucofrenatus, characterized by a larger amount of C-band positive segments spread over several...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Fish cytogenetics; Karyotypes; Ag-NORs; C-band; Chromosome evolution.
Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572006000100013
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Sympatric occurrence of two cytotypes of Astyanax scabripinnis (Characiformes, Characidae) Genet. Mol. Biol.
Maistro,Edson Luis; Oliveira,Claudio; Foresti,Fausto.
Among four specimens of Astyanax scabripinnis sympatrically collected from the Tamanduá stream (State of São Paulo, Brazil), three, named cytotype I, had 2n = 50 chromosomes (6M + 26SM + 4ST + 14A) and a fundamental number (FN) of 86, and one specimen, named cytotype II, had 2n = 48 chromosomes (6M + 28SM + 4ST + 10A) and FN = 86. Besides the difference in diploid number, the cytotypes showed a clear difference in the distribution of constitutive heterochromatin. One NOR-bearing chromosome pair was detected in both cytotypes and one specimen of cytotype I had multiple NORs (tree chromosome pairs). Robertsonian translocations and an increase or loss of heterochromatin are proposed to explain the karyotypic divergence observed. Some aspects related to the...
Tipo: Info:eu-repo/semantics/other
Ano: 2000 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572000000200021
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Cytogenetic analysis of three catfish species of the family Pseudopimelodidae (Teleostei, Siluriformes) Genet. Mol. Biol.
Martinez,Emanuel Ricardo Monteiro; Nirchio,Mauro; Granado,Angel; Foresti,Fausto; Oliveira,Claudio.
Cytogenetic analyses performed in Cephalosilurus apurensis, Microglanis aff. cottoides and Pseudopimelodus bufonius revealed that the three species have 2n = 54 chromosomes: C. apurensis presented six metacentric (M), 28 submetacentric (SM), 14 subtelolocentric (ST), and six acrocentric (A) chromosomes, while M. aff. cottoides showed 10M, 32SM, 10ST and 2A, and P. bufonius had 12M, 30SM and 12ST. The nucleolus organizer regions (NORs) were present on the short arm of a middle-sized ST pair, identified as pair 19, in C. apurensis NORs were found on the short arm of a middle-sized ST (pair 23) and on the long arm of a middle-sized ST (pair 22) in M. aff. cotttoides and on the short arm of three middle-sized ST pairs, identified as pairs 9, 10 and 11, in P....
Tipo: Info:eu-repo/semantics/article Palavras-chave: Karyotype; Chromosomes; C-banding; Ag-NOR; Fish.
Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572008000400015
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R- and G-band patterns in Astyanax scabripinnis paranae (Pisces, Characiformes, Characidae) Genet. Mol. Biol.
Maistro,Edson Luis; Foresti,Fausto; Oliveira,Claudio.
The absence of longitudinal bands in fish chromosomes has been associated with technical problems in chromosome preparations or the absence of a structural compartmentalization in the fish genome. In the present study, a R-banding pattern was obtained using a replication banding technique by in vivo treatment with 5-bromodeoxyuridine (5-BrdU). G-banding patterns were obtained after trypsin treatment and also after chromosome cleavage by in situ treatment with the restriction endonuclease BamHI. A similar G-banding pattern was also obtained after cleavage with the endonuclease HinfI. Presence of a resolute R- and G-banding patterns shows that Astyanax scabripinnis paranae chromosomes could present an isochore-like structure similar to that found in other...
Tipo: Info:eu-repo/semantics/article
Ano: 1999 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47571999000200011
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Cytogenetic characterization of the strongly electric Amazonian eel, Electrophorus electricus (Teleostei, Gymnotiformes), from the Brazilian rivers Amazon and Araguaia Genet. Mol. Biol.
Fonteles,Soraia B.A.; Lopes,Carlos E.; Akama,Alberto; Fernandes,Flora M.C.; Porto-Foresti,Fábio; Senhorini,José A.; Daniel-Silva,Maria de Fátima Z.; Foresti,Fausto; Almeida-Toledo,Lurdes Foresti de.
A karyotype analysis of the electric eel, Electrophorus electricus (Teleostei, Gymnotiformes), a strongly electric fish from northern South America, is presented. Two female specimens were analyzed, one from the Amazon River and one from the Araguaia River. The specimens had a chromosomal number of 2n = 52 (42M-SM + 10A). C-bands were present in a centromeric and pericentromeric position on part of the chromosomes; some interstitial C-bands were also present. Heteromorphic nucleolus organizer regions (NORs) were detected in two chromosome pairs of the specimen from the Amazon River. The chromosome number and karyotype characteristics are similar to those of other Gymnotidae species. The genera Electrophorus and Gymnotus are positioned as the basal lineages...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Chromosome; Fish; Gymnotidae; Electric eel.
Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572008000200010
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Analysis of the synaptonemal complex of the nine-banded armadillo, Dasypus novemcinctus Genet. Mol. Biol.
Scavone,Márcia Denise da Paixão; Oliveira,Claudio; Bagagli,Eduardo; Foresti,Fausto.
The synaptonemal complex (SC) of three specimens of the nine-banded armadillo (Dasypus novemcinctus) was analyzed. Thirty-two bivalents (2n = 64) were observed, 31 of them being autosomes and one an XY sexual bivalent. Chromosome synapsis processes and nucleolus structure changes were analyzed in zygotene and pachytene cells, allowing a detailed description of the beginning of meiotic prophase in this species. There was complete synapsis of X and Y chromosomes. Some abnormalities in SC were observed in cells during zygotene and at the beginning of pachytene, but not in cells in the middle and late pachytene, suggesting the occurrence of synaptic adjustments in their SC.
Tipo: Info:eu-repo/semantics/other
Ano: 2000 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572000000300020
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Cytogenetic analysis of five species of the subfamily Corydoradinae (Teleostei: Siluriformes: Callichthyidae) Genet. Mol. Biol.
Shimabukuro-Dias,Cristiane Kioko; Oliveira,Claudio; Foresti,Fausto.
In the present study, five callichthyid species belonging to the subfamily Corydoradinae were karyotyped: three species of Aspidoras and two of Corydoras. The three species of Aspidoras had the same diploid number, 2n = 46 chromosomes, similar karyotypic formulae, with most chromosomes metacentric or submetacentric, single interstitial Ag-NORs and C-band positive segments mainly found in the centromeric position. The comparative analysis of cytogenetic data available for the genus Aspidoras and other species of Corydoradinae suggest that several events of centric fusion occurred in the origin of the species of Aspidoras. The two analyzed species of Corydoras showed high diploid numbers, 2n = 74 in C. sodalis and 2n = 90 in C. britskii. While C. sodalis...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Fish cytogenetic; Karyotype; NOR; C-banding; Karyotypic evolution.
Ano: 2004 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572004000400014
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Cytogenetic characterization of F1, F2 and backcross hybrids of the Neotropical catfish species Pseudoplatystoma corruscans and Pseudoplatystoma reticulatum (Pimelodidae, Siluriformes) Genet. Mol. Biol.
Prado,Fernanda Dotti do; Nunes,Tatiana Leite; Senhorini,José Augusto; Bortolozzi,Jehud; Foresti,Fausto; Porto-Foresti,Fábio.
The cytogenetic characteristics of Pseudoplatystoma corruscans and Pseudoplatystoma reticulatum and their F1, F2 and backcross hybrids were assessed by using chromosome banding techniques. The diploid number of 56 chromosomes was constant in all species and lineages, with a karyotypic formula containing 20 metacentric, 12 submetacentric, 12 subtelocentric and 12 acrocentric chromosomes. Nucleolar organizer regions (NORs) were identified in two subtelocentric chromosomes in the parents and hybrids, with partial nucleolar dominance in F1 and F2 specimens. Heterochromatic blocks were detected in the terminal and centromeric regions of some chromosomes in all individuals. For parental and hybrid lineages, 18S ribosomal clusters corresponding to NORs and 5S...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Backcrossing; Cytogenetics; Interspecific hybrids; Post-F1 hybrids; Pseudoplatystoma.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572012000100008
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