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Jensen, Annette Nygaard; Storm, Christina; Forslund, Anita; Baggesen, Dorte Lau; Dalsgaard, Anders. |
In recent years, an increasing number of disease outbreaks have been associated with consumption of contaminated vegetables. Thus, it has been speculated to what extent such contamination is associated with application of animal manure as fertilizer, which is particularly practiced in organic vegetable production where conventional fertilizers are prohibited. A field survey was therefore performed aiming to assess the survival and transfer of E. coli from animal manure to lettuces, with E. coli serving as an indicator of bacterial enteric pathogens. Animal manure was applied to 3 Danish fields prior to planting of lettuce seedlings, then 5-8 weeks later at the normal time of harvest, inner and outer leafs of 10 lettuce heads were pooled into one sample... |
Tipo: Conference paper, poster, etc. |
Palavras-chave: Food security; Food quality and human health. |
Ano: 2010 |
URL: http://orgprints.org/20941/1/20941.pdf |
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Forslund, Anita; Plauborg, Finn; Andersen, Mathias N.; Markussen, Bo; Dalsgaard, Anders. |
The risk for contamination of potatoes and groundwater through subsurface drip irrigation with low quality water was explored in 30 large-scale lysimeters containing repacked coarse sand and sandy loam soils. The human pathogens, Salmonella Senftenberg, Campylobacter jejuni and E. coli O157:H7, and the virus indicator Salmonella Typhimurium bacteriophage 28B, were added weekly through irrigation tubes for one month with low irrigation rates (8 mm per week). In the following six months lysimeters were irrigated with groundwater free of pathogens. Two weeks after irrigation was started, phage 28B was detected in low concentrations (2 pfu ml-1) in leachate from both sandy loam soil and coarse sand lysimeters. After 27 days, phage 28B continued to be present... |
Tipo: Journal paper |
Palavras-chave: Food security; Food quality and human health Irrigation and drainage. |
Ano: 2011 |
URL: http://orgprints.org/18743/4/18743.pdf |
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Forslund, Anita; Markussen, Bo; Toenner-Klank, Lise; Bech, Tina B.; Jacobsen, Ole Stig; Dalsgaard, Anders. |
Increasing amounts of livestock manure are being applied to agricultural soil, but it is unknown to what extent this may be associated with contamination of aquatic recipients and groundwater if microorganisms are transported through the soil under natural weather conditions. The objective of this study was therefore to evaluate how injection and surface application of pig slurry on intact sandy clay loam soil cores influenced the leaching of Salmonella enterica serovar Typhimurium bacteriophage 28B, Escherichia coli, and Cryptosporidium parvum oocysts. All three microbial tracers were detected in the leachate on day 1, and the highest relative concentration was detected on the fourth day (0.1 pore volume). Although the concentration of the phage 28B... |
Tipo: Journal paper |
Palavras-chave: Food security; Food quality and human health Air and water emissions Irrigation and drainage. |
Ano: 2011 |
URL: http://orgprints.org/20435/4/20435.pdf |
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Jensen, Annette Nygaard; Storm, Christina; Forslund, Anita; Baggesen, Dorte Lau; Dalsgaard, Anders. |
A pilot study was conducted to assess the transfer of Escherichia coli from animal slurry fertilizer to lettuce, with E. coli serving as an indicator of fecal contamination and as an indicator for potential bacterial enteric pathogens. Animal slurry was applied as fertilizer to three Danish agricultural fields prior to the planting of lettuce seedlings. At harvest, leaves (25 g) of 10 lettuce heads were pooled into one sample unit (n ~ 147). Soil samples (100 g) were collected from one field before slurry application and four times during the growth period (n ~ 75). E. coli was enumerated in slurry, soil, and lettuce on 3M Petrifilm Select E. coli Count Plates containing 16 mg/liter streptomycin, 16 mg/liter ampicillin, or no antimicrobial agent. Selected... |
Tipo: Journal paper |
Palavras-chave: Food security; Food quality and human health Composting and manuring Crop husbandry Crop health; Quality; Protection. |
Ano: 2013 |
URL: http://orgprints.org/23106/7/23106.pdf |
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