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Inoculum padronization for the production of cutinase by Fusarium oxysporum BJM
Pio,Tatiana Fontes; Fraga,Laira Priscila; Macedo,Gabriela Alves.
Cutinase is a versatile enzyme showing several interesting properties for application in industrial processes. The widespread use of this enzyme depends on the development of an efficient and low-cost production system. One of the most important steps in a fermentation process is the standardization of the inoculum characteristics. In this study, the production of cutinase by Fusarium oxysporum showed a statistically significant relationship with both the inoculum size and the inoculum PDA pH. The greatest activities were 19.1 U/mL at PDA pH 7.0 and 22.72 U/mL using an aliquot of 12.72 x 10(7) spores/mL. The macroscopic characteristics of the colonies of Fusarium oxysporum changed according to the variation of the medium pH, with the best results recorded...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Fusarium oxysporum; Inoculum; Cutinase; Macroscopic characteristics; Fermentation process.
Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822008000100018
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Lipase and esterase: to what extent can this classification be applied accurately? Ciênc. Tecnol. Aliment.
Lopes,Danielle Branta; Fraga,Laira Priscila; Fleuri,Luciana Francisco; Macedo,Gabriela Alves.
Enzyme technology is an ever-growing field of knowledge and, in recent years, this technology has raised renewed interest, due to the search for new paradigms in several productive processes. Lipases, esterases and cutinases are enzymes used in a wide range of processes involving synthesis and hydrolysis reactions. The objective of this work was to investigate and compare the specific lipase and esterase activities of five enzymes - four already classified as lipases and one classified as cutinase - in the presence of natural and synthetic substrates. All tested enzymes presented both esterase and lipase specific activities. The highest specific esterase activity was observed for Aspergillus 1068 lipase in natural substrate and for F. oxysporum cutinase in...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Lipase; Esterase; Cutinase; Lipase specific activity; Esterase specific activity.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612011000300009
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