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	Provedor de dados Genet. Mol. Biol. (2) Autor Fregonezi,Jéferson Nunes (2) Vanzela,André Luís Laforga (2) Fernandes,Thiago (1) Fungaro,Maria Helena Pelegrinelli (1) Gaeta,Marcos Letaif (1) Torezan,José Marcelo Domingues (1) Vieira,Ana Odete S. (1) Vilas-Boas,Laurival A. (1) Mais... Palavra-chave CSRs (1) Cestrum (1) Cestrum intermedium (1) Cestrum strigilatum (1) Chromosome banding (1) Karyotype evolution (1) Karyotype organization (1) RDNA probes (1) Retroelements (1) Solanaceae (1) Ty3-gypsy (1) Mais... Tipo do documento Info:eu-repo/semantics/article (2) Ano 2007 (1) 2006 (1) País Brazil (2) Idioma Inglês (2)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 2 Primeira ... 1 ... Última Distribution of a Ty3/gypsy-like retroelement on the A and B-chromosomes of Cestrum strigilatum Ruiz & Pav. and Cestrum intermedium Sendtn. (Solanaceae) Genet. Mol. Biol. Fregonezi,Jéferson Nunes; Vilas-Boas,Laurival A.; Fungaro,Maria Helena Pelegrinelli; Gaeta,Marcos Letaif; Vanzela,André Luís Laforga. Retroelements are a diversified fraction of eukaryotic genomes, with the Ty1/copia and Ty3/gypsy groups being very common in a large number of plant genomes. We isolated an internal segment of the Ty3/gypsy retroelement of Cestrum strigilatum (Solanaceae) using PCR amplification with degenerate primers for a conserved region of reverse transcriptase. The isolated segment (pCs12) was sequenced and showed similarity with Ty3/gypsy retroelements of monocotyledons and dicotyledons. This segment was used as probe in chromosomes of C. strigilatum and Cestrum intermedium. Diffuse hybridization signals were observed along the chromosomes and more accentuated terminal signals in some chromosome pairs, always associated with nucleolus organizer regions (NORs). The... Tipo: Info:eu-repo/semantics/article Palavras-chave: Cestrum strigilatum; Cestrum intermedium; Karyotype organization; Retroelements; Solanaceae; Ty3-gypsy. Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000400017 Karyotype differentiation of four Cestrum species (Solanaceae) based on the physical mapping of repetitive DNA Genet. Mol. Biol. Fregonezi,Jéferson Nunes; Fernandes,Thiago; Torezan,José Marcelo Domingues; Vieira,Ana Odete S.; Vanzela,André Luís Laforga. We studied the karyotypes of four Brazilian Cestrum species (C. amictum, C. intermedium, C. sendtnerianum and C. strigilatum) using conventional Feulgen staining, C-Giemsa and C-CMA3/DAPI banding, induction of cold-sensitive regions (CSRs) and fluorescent in situ hybridization (FISH) with rDNA probes. We found that the karyotypes of all four species was 2n = 2x = 16, with, except for the eighth acrocentric pair, a predominance of meta- and submetacentric chromosomes and various heterochromatin classes. Heterochromatic types previously unreported in Cestrum as neutral C-CMA3(0)/DAPI0 bands, CMA3+ bands not associated with NORs, and C-Giemsa/CSR/DAPI- bands were found. The heterochromatic blocks varied in size, number, position and composition. The 45S rDNA... Tipo: Info:eu-repo/semantics/article Palavras-chave: Cestrum; Chromosome banding; CSRs; Karyotype evolution; RDNA probes. Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572006000100019 Registros recuperados: 2 Primeira ... 1 ... Última

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