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| Herzog-Soares,Joanna D'Arc A; Freire,Ronald Bastos. |
| Macrophages exposed to 10 mug/mL citrinin (CTR) or 0.01 mug CTR mixed with 0.04 mug aflatoxin B1 (AFB1) for a period of 2 h at 37ºC, were infected with 10(6) Toxoplasma gondii tachyzoites/muL. The parasites were treated with mycotoxins (2 h at 37ºC) before being added to the macrophage culture. The number of tachyzoites was quantified 2, 24, 48, 72 and 96 h after infection. During the first 2 hours, 59% infectivity was observed in the control. After exposure to CTR or the mixture of toxins (CTR-AFB1), macrophages were infected with 77.5% and 75% of the inoculated tachyzoites, respectively. Similarly, 72.3% of the cells were infected when cultured together with previously treated parasites. The treatment with CTR-AFB1 gave rise to 2.9 times more tachyzoites... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Aflatoxins; Imunossuspression; Macrophages; Citrinin; Toxoplasma gondii. |
| Ano: 2004 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702004000100007 |
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| Freire,Ronald Bastos; Freire,Márcia de Fátima Inácio; Berbara,Ricardo Louro. |
| O presente experimento descreve, pela primeira vez, a elaboração de um ensaio imunoenzimático (ELISA de captura) com anticorpos IgY para a detecção e quantificação de acetato de lupeol (LAc) em Vernonia scorpioides Lam. Pers (Asteraceae). Anticorpos anti-LAc, obtidos partir do soro das aves e gemas de seus ovos, após imunização com o conjugado acetato de lupeol-cBSA, foram concentrados em coluna contendo LAc-BSA acoplado a sepharose. A eficácia da metodologia de detecção imunológica foi de 97%, com índices de sensibilidade e especificidade de 99% e 95%, respectivamente com limites de detecção do ensaio entre 0,02µg.g-1 (inferior) e 10 µg.g-1 (superior). A robustez do método foi atestada pela sua elevada reprodutibilidade (entre 94,75% e 96,81%), e pelo... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: IgY-ELISA; IgY; Vernonia scorpioides; Acetato de lupeol; Controle de qualidade vegetal. |
| Ano: 2004 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782004000400016 |
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| Barbosa,Celso Guimarães; Sanavria,Argemiro; Freire,Ronald Bastos. |
| Six bovines were infested with 60 first instar larvae of Dermatobia hominis. The animals were bleed weekly, and their antibodies levels to D. hominis L1, L2 and L3 instars measured during the time, following the infestation course. The antisera were submitted to a titration against optimal dilutions of antigen coated wells of microplates, previously sensitized with L1, L2 and L3 preparations, respectively. The ELISA assay was used to test single dilutions of antisera, which results were comparatively analyzed with a control of not infested animals. Antibodies against L1 were detected between the first and 21st day post-infestation (DPI) and, from the 42nd DPI on. Anti-L2 antibodies, could be detected on the 21st DPI and from the 35th DPI until... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Dermatobia hominis; Myiasis; Humoral immune response; ELISA. |
| Ano: 2000 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782000000300013 |
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