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Carvalho,Claudemir de; Freitas,Antonio Carlos de; Brunner,Olga; Góes,Luiz Gustavo Bentim; Cavalcante,Andréa Yaguiu; Beçak,Willy; Santos,Rita de Cassia Stocco dos. |
Papillomaviruses are described selectively infecting epithelial tissues and are associated with many forms of cancer in different species. Considering the widespread dissemination of papillomatosis in livestock, interest is being centred on possible forms of viral transmission and respective mechanisms. In the present study, we report the detection of bovine papillomavirus (BPV) DNA sequences in female reproductive tract tissues, fluids and oocytes from slaughtered bovines not afflicted by cutaneous papillomatosis. BPV-2 DNA sequences were found in ovarian and uterine tissues as well as in oocytes, cumulus cells and uterine flushings. The presence of papillomavirus sequences in reproductive organ tissues and fluids shows that viral infection in organisms... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: BPV-2; Papillomatosis; Gametes; PCR. |
Ano: 2003 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822003000500028 |
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Góes,Luiz Gustavo Bentim; Freitas,Antonio Carlos de; Ferraz,Oilita Pereira; Rieger,Tania Tassinari; Santos,José Ferreira dos; Pereira,Alexandre; Beçak,Willy; Lindsey,Charles J.; Stocco,Rita de Cassia. |
The development of a bovine papillomavirus (BPV) vaccine is an outstanding challenge. BPV protein L1 gene transfection in the Drosophila melanogaster S2 cell expression system failed to produce L1 protein notwithstanding correct L1 gene insertion. Severe genetic inbalance in the host cell line, including cytogenetic alterations, may account for the lack of protein expression. |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Bovine Papillomavirus; BPV4; S2 expression cells. |
Ano: 2008 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822008000100001 |
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Freitas,Antonio Carlos de; Carvalho,Claudemir de; Brunner,Olga; Birgel-Junior,Eduardo Harry; Dellalibera,Alice Maria Melville Paiva; Benesi,Fernando José; Gregory,Lilian; Beçak,Willy; Santos,Rita de Cassia Stocco dos. |
This study has detected BPV-1 DNA sequences in wart, blood and plasma samples collected from animals affected by papillomatosis, suggesting viral presence inside the cell. We sellected an animal in which we could detect BPV-1 DNA sequences in wart, blood, placenta and amniotic liquid samples and her offspring which presented BPV-1 DNA sequences in blood sample collected immediately after birth. These results show a possible vertical transmission of BPV-1. |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: BPV; Viral vertical transmission; PCR detection. |
Ano: 2003 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822003000500026 |
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