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| Registros recuperados: 14 | |
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| Silva-Zacarias,Francielle Gibson da; Alfieri,Amauri Alcindo; Spohr,Kledir Anderson Hofstaetter; Lima,Bruna Azevedo de Carvalho; Negrão,Fábio Juliano; Lunardi,Michele; Freitas,Julio Cesar de. |
| Chlamydophila abortus (C. abortus) is associated with reproductive problems in cattle, sheep, and goats. Diagnosis of C. abortus using embryonated chicken eggs or immortalized cell lines has a very low sensitivity. Polymerase chain reaction (PCR) assays have been used to detect C. abortus infection in clinical specimens and organ fragments, such as placenta, fetal organs, vaginal secretions, and semen. The aim of this study was to develop a PCR assay for the amplification of an 856-bp fragment of the rRNA gene of the Chlamydiaceae family. The PCR assay was evaluated using organs from 15 mice experimentally infected with the S26/3 reference strain of C. abortus. The results of the rRNA PCR were compared to the results from another PCR system (Omp2 PCR) that... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Bovine; Ovine; Caprine; Reproductive failures; Chlamydophila abortus; PCR. |
| Ano: 2009 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000700014 |
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| Silva-Zacarias,Francielle Gibson da; Alfieri,Amauri Alcindo; Spohr,Kledir Anderson Hofstaetter; Lima,Bruna Azevedo de Carvalho; Oliveira,Rosângela Claret de; Turilli,Carlo; Lunardi,Michele; Otonel,Rodrigo Alejandro Arellano; Freitas,Julio Cesar de. |
| Chlamydophila abortus (C. abortus) infection is related to reproductive failure in domestic ruminants. Although it has not been well characterized worldwide, this pathogen has already been identified in some European countries and in the USA. In Brazil, preliminary studies have shown serological evidence of C. abortus infection in herds with low antibody prevalence. Until now, the identification of C. abortus in biological samples from females presenting reproductive failures has not been described in Brazilian herds of domestic ruminants. The aim of this study was to evaluate the presence of the C. abortus in a collection of abortions from cattle (n=85), sheep (n=12), and goats (n=8), in samples of vaginal mucus from cows (n=13), sheep (n=90), and goats... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Cattle; Small ruminants; Chlamydophila abortus; Abortion; Vaginal mucus; Semen. |
| Ano: 2009 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000700015 |
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| Delbem,Ádina Cléia Botazzo; Freitas,Julio Cesar de; Bracarense,Ana Paula F. R. L.; Müller,Ernst Eckehardt; Oliveira,Rosângela Claret de. |
| Swine leptospirosis is recognized world wide as an important cause of reproductive failure. Serum and histo-pathological examinations have been constantly used to diagnose this disease. This study was carried out on slaughtered sows from Northern Paraná State to compare serum findings with kidney, liver, ovary and uterus histo-pathological examinations. The microscopic serum-agglutination carried out on 36 animals detected 24 with titles > 100 and 12 negative (< 100). The histopathological examinations made on livers, kidneys, ovaries and uterus stained with hematoxylin-eosin, were carried out on 16 randomly chosen animals, 11 from the groups with > 100 serological titles and five from the negative group. The results showed that all the 16 animals... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Leptospira; Swine; Diagnostic. |
| Ano: 2002 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822002000200016 |
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| Gonçalves,Daniela Dib; Benitez,Aline; Lopes-Mori,Fabiana Maria Ruiz; Alves,Lucimara Aparecida; Freire,Roberta Lemos; Navarro,Italmar Teodorico; Santana,Maria Aparecida Zanella; Santos,Luís Roberto Alves dos; Carreira,Teresa; Vieira,Maria Luísa; Freitas,Julio Cesar de. |
| The aim of this study was to conduct a serological survey for Lyme diseases, brucellosis, leptospirosis and toxoplasmosis and identify the risk variables related to these zoonoses in humans living in the rural area of Jataizinho, state of Parana, Brazil. A total of 63 rural properties were surveyed. Additionally, 207 serum samples collected from these rural area inhabitants were tested for indirect immunofluorescence (IFI) and western blots (WB) were performed to detect Borrelia burgdorferi (sensu lato); a tamponated acidified antigen test (AAT) and 2-mercaptoethanol (2-ME) were used to detect antibodies of Brucella abortus; the microscopic agglutination test (MAT) was carried out to detect antibodies anti-Leptospira spp. and IFI was used to find... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Lyme disease; Leptospirosis; Brucellosis; Toxoplasmosis; Human; Antibody. |
| Ano: 2013 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822013000100018 |
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| Silva,Francielle Gibson da; Freitas,Julio Cesar de; Anzai,Eleine Kuroki; Hashimoto,Vanessa Yumi; Giraldi,Nilson; Delbem,Ádina Cléia B.; Bracarense,Ana Paula F.R.L.; Reis,Antônio Carlos F. dos; Vasconcellos,Silvio Arruda. |
| The objective of this trial was to detect leptospires in fragments of kidney, liver and uterus of 96 cows with unknown sanitary status, randomly chosen at slaughter in Paraná, Brazil. All 96 urine samples were submitted to direct examination using dark field microscopy. Positive samples in the direct examination and all kidney, liver and uterus fragments were cultured in EMJH modified medium and Tween 80/40/LH. Sections cut of kidney, liver and uterus were stained by Hematoxylin-Eosin (HE) and indirect immunoperoxidase with hyperimmune serum against serovar Hardjo (Hardjoprajitno) and strain Londrina 14 (LO14), from serovar Canicola, which was isolated in the northern region of Paraná state, Brazil, used as primary antibodies. Direct examination detected... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Leptospire; Indirect immunoperoxidase; Immunohistochemistry; Cows; Culture. |
| Ano: 2005 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822005000100008 |
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| Gonçalves,Daniela Dib; Carreira,Teresa; Nunes,Mónica; Benitez,Aline; Lopes-Mori,Fabiana Maria Ruiz; Vidotto,Odilon; Freitas,Julio Cesar de; Vieira,Maria Luísa. |
| The aim of this study was to investigate the presence of DNA of Borrelia burgdorferi sensu lato (s.l.) in ticks that feed on horses used for animal traction in rural Jataizinho, Parana, Brazil. Between February and June 2008, a total of 224 ticks was collected of which 75% were identified as Dermacentor nitens and 25% as Amblyomma cajenense. To amplify B. burgdorferi s.l. DNA, the intergenic space region (ISR) between the 5S (rrf) 23S (rrl) rRNA genes was used as targets for nested-PCR. Two ticks of the D. nitens species were positive for B. burgdorferi s.l. Both species showed a fragment of 184 bp, but the sequencing revealed 99.9% homology with the B. burgdorferi sensu stricto (s.s.) strain B31. These results showed, for the first time, the presence of... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Ticks; Dermacentor nitens; Borrelia burgdorferi sensu lato; Equine; Rural area. |
| Ano: 2013 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822013000300035 |
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| Rodrigues,Cibele Giatti; Müller,Ernst Eckehardt; Freitas,Julio Cesar de. |
| Uma avaliação sorológica para 22 sorotipos de L. interrogans foi realizada. Usou-se o teste de microaglutinação rápida no soro de 1253 fêmeas bovinas, adultas de 14 propriedades produtoras de leite tipo B, da região de Londrina-Paraná- Brasil, onde havia animais com problemas reprodutivos. Títulos de anticorpos > 100 foram detectados em 166 (13,25%) animais de 10 (71,43%) propriedades. Em 56 (33,73%) animais foram encontrados anticorpos contra dois ou mais sorotipos simultaneamente. Anticorpos contra o sorotipo icterohaemorrhagiae foram detectados em 48 (28,91%) animais, seguido pelos sorotipos pomona 35 (21,08%); bataviae 28 (16,87%); autumnalis 24 (14,46%); canicola 19 (11,44%); hardjo 18 (10,84%); bratislava 17 (10,24%); butembo 13 (7,83%); pyrogenes... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Bovino; Leptospira interrogans; Sorologia. |
| Ano: 1999 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84781999000200020 |
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| Laffranchi,Alessandra; Müller,Ernst Eckehardt; Freitas,Julio Cesar de; Pretto-Giordano,Lucienne Garcia; Dias,Juliana Alves; Salvador,Rogério. |
| De 88 vacas primíparas, oriundas de quatro rebanhos leiteiros, foram colhidas 1985 amostras de leite, ao longo dos 120 dias pós-parto, das quais 457 (23,02%) apresentaram resultados microbiológicos positivos. Os estafilococos coagulase negativos (ECN) foram isolados em 316 (69,14%) amostras, Corynebacterium bovis em 56 (12,25%), estreptococos em 41 (8,97%) e estafilococos coagulase positivos (ECP) em 38 (8,31%). Mastite clínica foi detectada em nove (10,23%) vacas. No primeiro dia pós-parto, 57 (64,77%) animais e 114 (32,66%) quartos apresentaram exames bacteriológicos positivos. Até o décimo quarto dia, ocorreu um decréscimo acentuado no número de vacas e quartos infectados, que posteriormente tendeu a estabilizar. Os ECN foram as bactérias mais isoladas... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Mastite; Etiologia; Vacas primíparas. |
| Ano: 2001 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782001000600018 |
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| Freitas,Julio Cesar de; Silva,Francielle Gibson da; Oliveira,Rosângela Claret de; Delbem,Ádina Cléia Botazzo; Müller,Ernst Eckehardt; Alves,Lucimara Aparecida; Teles,Paulo Sergio. |
| Leptospira isolation allows definitive diagnosis of the infection. Contamination by microorganisms is one of the inconveniences of the culture. The objective of this study was to describe the isolation of Leptospira from dogs, bovine and swine naturally infected. Urine samples from 14 dogs and three bovines, and kidney, liver, ovary, and uterus body samples from 36 slaughtered sows with unknown health history, were used. The urine and organ samples were cultured in culture medium. Modified Ellinghausen-McCullough-Johnson-Harris medium (EMJH) culture medium was used with addition of 5-fluorouracil, chloramphenicol, vancomycin, nalidixic acid and neomycin. Incubation was performed at 28oC for 24 hours, followed by subculture in modified EMJH without... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Leptospirosis; Diagnostic; Culture; Cattle; Dog; Swine. |
| Ano: 2004 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782004000300030 |
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| Delbem,Ádina Cléia Botazzo; Freire,Roberta Lemos; Silva,Caio Abércio da; Müller,Ernst Eckehardt; Dias,Ricardo Augusto; Ferreira Neto,José Soares; Freitas,Julio Cesar de. |
| A leptospirose é uma zoonose amplamente difundida e de grande importância sócio-econômica para a suinocultura. Com o objetivo de individualizar condutas de manejo ou condições ambientais que estejam relacionadas ao risco de infecção por leptospirose, foram estudadas 298 matrizes suínas provenientes de granjas da região norte do Estado do Paraná. Todos os animais foram submetidos à prova de soroaglutinação microscópica e para cada um deles foi aplicado um questionário epidemiológico, que procurou investigar variáveis que poderiam estar associadas a essa infecção. Os resultados obtidos foram submetidos ao teste c², seguido de análise multivariada. Foram detectados títulos de anticorpos >100 em 132 matrizes suínas (44,3%), sendo em maior freqüência contra... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Leptospirose; Matrizes suínas; Fatores de risco; Diagnóstico. |
| Ano: 2004 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782004000300029 |
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| Silva,Francielle Gibson da; Freitas,Julio Cesar de; Müller,Ernst Eckehardt. |
| A Chlamydophila abortus (anteriormente classificada como Chlamydia psittaci sorotipo 1) tem sido descrita em muitos países, associada principalmente com distúrbios reprodutivos em ovinos, bovinos e caprinos. O aborto enzoótico dos ovinos e caprinos e o aborto epizoótico dos bovinos são as doenças mais importantes causadas por esta bactéria. No Brasil, as pesquisas com C. abortus são praticamente inexistentes. O objetivo desta revisão é apresentar informações sobre modificações taxonômicas, ciclo de vida, epidemiologia, patogenia, sinais clínicos e diagnóstico da infecção por C. abortus principalmente em ovinos, bovinos e caprinos. |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Chlamydophila abortus; Ovino; Bovino; Caprino; Aborto; Problema reprodutivo. |
| Ano: 2006 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782006000100057 |
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| Dreer,Márcia Küster de Paula; Gonçalves,Daniela Dib; Caetano,Isabel Cristina da Silva; Gerônimo,Edson; Menegas,Paulo Henrique; Bergo,Danilo; Lopes-Mori,Fabiana Maria Ruiz; Benitez,Aline; Freitas,Julio Cesar de; Evers,Fernanda; Navarro,Italmar Teodorico; Martins,Lisiane de Almeida. |
| Background : Leptospirosis, toxoplasmosis and brucellosis are diseases with worldwide distribution. Among stray dogs, these zoonoses are facilitated by direct contact with other animal species, by the habit of scavenging garbage and hunting in search of food, drinking standing water, smelling other animals' urine, licking female genitalia and the sexual act itself. The objective of this study was to detect antibodies anti-Toxoplasma gondii, anti-Leptospira spp., anti-Brucella canis and anti-Brucella abortus in stray dogs housed in shelters at Umuarama city, Paraná, Brazil. In order to detect toxoplasmosis, indirect immunofluorescence assay (IFA) was performed, agglutination microscopic (MAT) test for leptospirosis and agar gel immunodiffusion (AGID) and... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Leptospira spp; Brucella abortus; Brucella canis; Toxoplasma gondii; Zoonosis. |
| Ano: 2013 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992013000100313 |
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| Registros recuperados: 14 | |
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