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| Pereira,Mayra Ramos de Jesus; Pinhatti,Valéria Rodrigues; Silveira,Maiele Dornelles da; Matzenbacher,Cristina Araujo; Freitas,Thales Renato Ochotorena de; Silva,Juliana da; Camassola,Melissa; Nardi,Nance Beyer. |
| Abstract Mesenchymal stem/stromal cells (MSCs) are multipotent cells distributed in all tissues and characterized by adherence, morphology, immunophenotype and trilineage differentiation potential. The present study aimed to isolate and characterize adherent MSC-like populations from different tissues of Ctenomys minutus, a threatened wildlife rodent popularly known as tuco-tuco. Adherent cells were isolated from bone marrow, brain, liver, pancreas and adipose tissue of three adult animals collect in southern Brazil. Cultures showed typical morphology and proliferation potential. Adipose-derived MSCs showed trilineage potential. Cultures derived from adipose tissue, bone marrow and brain were immunophenotyped with negative results for CD31, CD44, CD45,... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Mesenchymal stem/stromal cells; Tuco-tuco; Ctenomys minutus. |
| Ano: 2018 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572018000500870 |
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| Bülau,Sandra Eloisa; Kretschmer,Rafael; Gunski,Ricardo José; Garnero,Analía del Valle; O’Brien,Patricia C. M.; Ferguson-Smith,Malcolm A.; Oliveira,Edivaldo Herculano Correa de; Freitas,Thales Renato Ochotorena de. |
| Abstract Zonotrichia capensis is widely distributed in the Neotropics. Previous cytogenetic studies demonstrated the presence of polymorphisms in two chromosome pairs (ZCA2 and ZCA4). Here, we report results based on comparative chromosome painting, using probes derived from Gallus gallus and Leucopternis albicollis, focused on characterizing the chromosome organization of Z. capensis. Our results demonstrate the conservation of ancestral syntenies as observed previously in other species of passerine. Syntenies were rearranged by a series of inversions in the second chromosome as described in other Passeriformes, but in this species, by using probes derived from L. albicollis we observed an extra inversion in the second chromosome that had not previously... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Birds; Chromosomal rearrangements; Molecular cytogenetics; FISH. |
| Ano: 2018 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572018000500799 |
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| Tchaicka,Ligia; Freitas,Thales Renato Ochotorena de; Bager,Alex; Vidal,Stela Luengos; Lucherini,Mauro; Iriarte,Agustín; Novaro,Andres; Geffen,Eli; Garcez,Fabricio Silva; Johnson,Warren E.; Wayne,Robert K.; Eizirik,Eduardo. |
| Abstract To investigate the evolution and biogeography of an endemic group of South American foxes, we examined mitochondrial DNA control region sequences for 118 individuals belonging to all six extant species of the genus Lycalopex. Phylogenetic and molecular dating analyses supported the inference that this genus has undergone a very recent and rapid radiation, stemming from a common ancestor that lived ca. 1 million years ago. The Brazilian endemic L. vetulus was supported as the most basal species in this genus, whereas the most internal group is comprised by the recently diverged (ca. 350,000 years ago) Andean/Patagonian species L. griseus and L. culpaeus. We discuss the inferred phylogenetic relationships and divergence times in the context of the... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Mitochondrial DNA control region; Lycalopex; Canidae; Carnivora. |
| Ano: 2016 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572016000300442 |
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| Kretschmer,Rafael; Souza,Marcelo Santos de; Barcellos,Suziane Alves; Degrandi,Tiago Marafiga; Pereira,Jorge C.; O’Brien,Patricia C.M.; Ferguson-Smith,Malcolm A.; Gunski,Ricardo José; Garnero,Analía del Valle; Oliveira,Edivaldo Herculano Correa de; Freitas,Thales Renato Ochotorena de. |
| Abstract The order Charadriiformes comprises three major clades: Lari and Scolopaci as sister group to Charadrii. Until now, only three Charadriiformes species have been studied by chromosome painting: Larus argentatus (Lari), Burhinus oedicnemus and Vanellus chilensis (Charadrii). Hence, there is a lack of information concerning the third clade, Scolapaci. Based on this, and to gain a better understanding of karyotype evolution in the order Charadriiformes, we applied conventional and molecular cytogenetic approaches in a species belonging to clade Scolopaci - the wattled jacana (Jacana jacana) - using Gallus gallus and Zenaida auriculata chromosome-specific probes. Cross-species evaluation of J. jacana chromosomes shows extensive genomic reshuffling... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Charadrii; Karyotype; Avian genome; Comparative mapping. |
| Ano: 2020 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572020000100601 |
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