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Development of a polymerase chain reaction for the detection of abalone herpesvirus infection based on the DNA polymerase gene ArchiMer
Chen, M.h.; Kuo, S. T.; Renault, Tristan; Friedman, C. S.; Chang, P. H..
A 5781-base pair (bp) fragment of genomic DNA from the Taiwanese abalone herpesvirus was obtained and showed 99% (5767/5779) homology in the nucleotide sequence and 99% (1923/1926) in the amino acid sequence with the DNA polymerase gene of the abalone herpesvirus strain Victoria/AUS/2007. Homology of the amino acid sequence with the DNA polymerase of ostreid herpesvirus 1 was 30% (563/1856). In this study, a PCR-based procedure for detecting herpesvirus infection of abalone, Haliotis diversicolor supertexta, in Taiwan was developed. The method employed primer sets targeting the viral DNA polymerase gene, and was able to amplify DNA fragments of the expected size from infected samples. Primer sets of 40f and 146r were designed for amplification of an...
Tipo: Text Palavras-chave: Herpes virus; Abalone; Haliotis diversicolor supertexta; DNA polymerase; Polymerase chain reaction.
Ano: 2012 URL: http://archimer.ifremer.fr/doc/00098/20952/19675.pdf
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