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Biological processes dominate phosphorus dynamics under low phosphorus availability in organic horizons of temperate forest soils Organic Eprints
Pistocchi, Chiara; Mészáros, Éva; Tamburini, Federica; Frossard, Emmanuel; Bünemann, Else Katrin.
Understanding the mechanisms underlying phosphorus (P) availability is important to predict forest productivity in a changing environment. We quantified P fluxes and traced P from plant litter into inorganic and organic soil P pools in organic horizons from two contrasting temperate forest soils with low and high inorganic P availability, respectively. We incubated the two organic horizons with and without litter after labelling the soil solution with 33P and performed sequential extractions at several time points in order to trace P dynamics in labile (water-extractable, available and microbial P) and non-labile (non-living organic P, P bound to iron and aluminium and P bound to calcium) pools. Under low P availability, P fluxes were dominated by gross P...
Tipo: Journal paper Palavras-chave: Soil quality.
Ano: 2018 URL: http://orgprints.org/34708/1/Pistocchi-etal-2018-SoilBiolBiochem-Vol126-p64-75.pdf
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Soil bacterial and fungal communities along a soil chronosequence assessed by fatty acid profiling Organic Eprints
Welc, Monika; Bünemann, Else K.; Fließbach, Andreas; Frossard, Emmanuel; Jansa, Jan.
Microbial communities are important components of terrestrial ecosystems. The importance of their diversity and functions for natural systems is well recognized. However, a better understanding of successional changes of microbial communities over long time scales is still required. In this work, the size and composition of microbial communities in soils of a deglaciation chronosequence at the Damma glacier forefield were studied by fatty acid profiling. Soil fatty acid concentrations clearly increased with soil age. The abundances of arbuscular mycorrhizal fungi (AMF), bacteria and other soil fungi, however, were more affected by abiotic soil parameters like carbon content and pH than by soil age. Analysis of ratios of the different microbial groups (AMF,...
Tipo: Journal paper Palavras-chave: Soil quality.
Ano: 2012 URL: http://orgprints.org/21946/1/welc-etal-2012-SoilBioloBiochemistry-49-p184-192.pdf
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Total and active microbial communities and phoD as affected by phosphate depletion and pH in soil Organic Eprints
Ragot, Sabine A.; Huguenin-Elie, Olivier; Kertesz, Michael A.; Frossard, Emmanuel; Bünemann, Else K..
Background and Aims Soil microbial communities contribute to organic phosphorus cycling in a variety of ways, including secretion of the PhoD alkaline phosphatase. We sampled a long-term grassland fertilization trial in Switzerland characterized by a natural pH gradient. We examined the effects of phosphate depletion and pH on total and active microbial community structures and on the structure and composition of the total and active phoD-harboring community. Methods Archaeal, bacterial and fungal communities were investigated using T-RFLP and phoD-harboring members of these communities were identified by 454-sequencing. Results Phosphate depletion decreased total, resin-extractable and organic phosphorus and changed the structure of all active...
Tipo: Journal paper Palavras-chave: Soil quality.
Ano: 2016 URL: http://orgprints.org/32321/1/ragot-etal-2017-PlantSoil-Vol408-Issue1-2-p15-30.pdf
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Phosphorus forms and enzymatic hydrolyzability of organic phosphorus in soils after 30 years of organic and conventional farming Organic Eprints
Keller, Martina; Oberson, Astrid; Annaheim, Kathrin E.; Tamburini, Federica; Mäder, Paul; Mayer, Jochen; Frossard, Emmanuel; Bünemann, Else K..
Lower P-input levels in organic than conventional farming can decrease soil total and available P, which can potentially be resupplied from soil organic P. We studied the effect of 30 y of conventional and organic farming on soil P forms, focussing especially on organic P. Soil samples (0–20 cm) were taken in a field experiment with a nonfertilized control, two organic systems receiving P inputs as animal manure, and two conventional systems receiving only mineral P or mineral P and manure. Soils were analyzed for total, inorganic, organic, and microbial P, by sequential P fractionation and by enzyme additions to alkaline soil extracts. Samples taken prior to starting the experiment were also analyzed. Average annual P balances ranged from –20 to +5 kg...
Tipo: Journal paper Palavras-chave: Soil quality; Crop husbandry.
Ano: 2012 URL: http://orgprints.org/21783/1/keller-etal-2012-JPlantNutrSoilSci-Vol175-p385-393.pdf
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Einfluss unterschiedlicher Bewirtschaftungsverfahren auf P-Formen und P-Dynamik im Boden Organic Eprints
Keller, Martina; Oberson, Astrid; Frossard, Emmanuel; Mäder, Paul; Mayer, Jochen; Bünemann, Else K..
The impact of cropping systems on soil phosphorus (P) forms and P dynamics was studied. The systems were a biodynamic (DYN), a bioorganic (BIO), a conventional (CON), a stockless system (MIN) and an unfertilized control (NON). These cropping systems are part of the DOK field trial in Therwil near Basel. The systems differed in fertilization and plant protection. The P budgets over 30 years were negative except for CON and increased in the order: NON < DYN < ORG < MIN < CON. A sequential P extraction scheme was used to characterize the P forms in soil samples from 2007 and 1977 (before the start of the trial). Labile inorganic P (Pi) forms differed significantly between cropping systems. Organic P forms (Po) and stable Pi forms were not...
Tipo: Conference paper, poster, etc. Palavras-chave: Nutrient turnover; Soil; Composting and manuring.
Ano: 2009 URL: http://orgprints.org/14255/1/Keller_14255.pdf
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Identification of Heterotrophic Zinc Mobilization Processes among Bacterial Strains Isolated from Wheat Rhizosphere (Triticum aestivum L.) Organic Eprints
Costerousse, Benjamin; Schönholzer-Mauclaire, Laurie; Frossard, Emmanuel; Thonar, Cécile.
Soil and plant inoculation with heterotrophic zinc-solubilizing bacteria (ZSB) is considered a promising approach for increasing zinc (Zn) phytoavailability and enhancing crop growth and nutritional quality. Nevertheless, it is necessary to understand the underlying bacterial solubilization processes to predict their repeatability in inoculation strategies. Acidification via gluconic acid production remains the most reported process. In this study, wheat rhizosphere soil serial dilutions were plated on several solid microbiological media supplemented with scarcely soluble Zn oxide (ZnO), and 115 putative Zn-solubilizing isolates were directly detected based on the formation of solubilization halos around the colonies. Eight strains were selected based on...
Tipo: Journal paper Palavras-chave: Cereals; Pulses and oilseeds Nutrient turnover Crop health; Quality; Protection.
Ano: 2018 URL: http://orgprints.org/32662/1/Costerousse-etal-2018-ApplEnvironMicrobiol-vol84-issue1-p1-16.pdf
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Nitrogen Budgets and Soil Nitrogen Stocks of Organic and Conventional Cropping Systems: Trade-Off between Efficiency and Sustainability of Nitrogen Use Organic Eprints
Oberson, Astrid; Jarosch, Klaus A.; Bosshard, Christine; Dubois, David; Frossard, Emmanuel; Hammelehle, Andreas; Mäder, Paul; Mayer, Jochen.
Organic and conventional cropping systems differ in the nature and amounts of nitrogen (N) inputs, which may affect efficiency and sustainability of N use. In the DOK (bio-Dynamic, bio-Organic, Konventionell) field experiment, organic and conventional cropping systems have been compared since 1978 at two fertilization levels. Nitrogen inputs via manure and/or mineral fertilizers, and N exports from plots with harvested products have throughout been recorded. For all treatments, N outputs with harvests have exceeded the inputs with fertilizers. Over the past years, symbiotic N2 fixation by soybean and clover grown in the trial has additionally been assessed, indicating average annual inputs of about 100 kg ha-1 yr-1 of N fixed from the atmosphere. Soil...
Tipo: Conference paper, poster, etc. Palavras-chave: "Organics" in general; Soil quality; Soil tillage.
Ano: 2018 URL: http://orgprints.org/34559/1/oberson-etal-2018ESA2018_FinalAbstractBook_p44.pdf
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Positive Mischungseffekte auf Ertrag und Stickstoffversorgung in Klee-Gras-Mischungen Organic Eprints
Huguenin-Elie, Olivier; Nyfeler, Daniel; Suter, Matthias; Frossard, Emmanuel; Lüscher, Andreas.
High plant species richness has been shown to positively influence grassland biomass production. In mixtures containing two forage grass and two forage legume species we quantified the diversity-productivity effect and the nitrogen economy of the swards across a broad range of species proportions. Positive diversity-productivity effects resulted in higher yield (up to +35%) of the four-species grass-clover-mixtures than the highest yielding monoculture (transgressive overyielding). The diversity-productivity effect depended on the species proportion in the mixture and was maximal in well balanced mixtures with a clover proportion of 40 – 60%. Transgressive overyielding was significant over a fairly wide range of clover proportions. Well balanced...
Tipo: Conference paper, poster, etc. Palavras-chave: Pasture and forage crops.
Ano: 2009 URL: http://orgprints.org/14416/1/HugueninElie_14416.pdf
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Tracing the fate of 15N-labelled animal manure in the environment Organic Eprints
Frick, Hanna; Oberson, Astrid; Frossard, Emmanuel; Wettstein, Hans-Rudolf; Bünemann, Else K..
Background • Nitrate leaching threatens both the environment and drinking water quality • Switzerland ca. 380,000 ha with > 25 mg nitrate/L in groundwater (quality criteria for drinking water) [1]; the Gäu region (Canton Solothurn) is especially vulnerable • Organic fertilizers (slurry, farmyard manure, etc.) might entail considerably increased leaching risks due to variable nitrogen (N) content and availability • Aim: increase N use efficiency, simultaneously reduce leaching
Tipo: Conference paper, poster, etc. Palavras-chave: Composting and manuring; Air and water emissions.
Ano: 2018 URL: http://orgprints.org/34860/1/Poster_Hanna_A0_1806_light.pdf
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Long-term organic matter application reduces cadmium but not zinc concentrations in wheat Organic Eprints
Grüter, Roman; Costerousse, Benjamin; Mayer, Jochen; Mäder, Paul; Thonar, Cécile; Frossard, Emmanuel; Schulin, Rainer; Tandy, Susan.
Wheat is a staple food crop and a major source of both the essential micronutrient zinc (Zn) and the toxic heavy metal cadmium (Cd) for humans. Since Zn and Cd are chemically similar, increasing Zn concentrations in wheat grains (biofortification), while preventing Cd accumulation, is an agronomic challenge. We used two Swiss agricultural long-term field trials, the “Dynamic-Organic-Conventional System Comparison Trial” (DOK) and the “Zurich Organic Fertilization Experiment” (ZOFE), to investigate the impact of long-term organic, mineral and combined fertilizer inputs on total and phytoavailable concentrations of soil Zn and Cd and their accumulation in winter wheat (Triticum aestivum L.). “Diffusive gradients in thin films” (DGT) and...
Tipo: Journal paper Palavras-chave: Soil quality Cereals; Pulses and oilseeds Composting and manuring.
Ano: 2019 URL: http://orgprints.org/36388/1/grueter-etal-2019-SciTotalEnv-Vol669-p608-620.pdf
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Fresh and residual phosphorus uptake by ryegrass from soils with different fertilization histories Organic Eprints
Oberson, Astrid; Tagmann, Hans U.; Langmeier, Monika; Dubois, David; Mäder, Paul; Frossard, Emmanuel.
Organic farming largely depends on animal manure as a source of phosphorus (P) and the recycling of animal manure globally is becoming increasingly important. In a pot experiment, using radioactive P labeling techniques, we studied ryegrass uptake of P applied with animal manure and water soluble mineral fertilizer to soils that had been cropped for 22 years according to organic or conventional farming practices. The soils differed in P status and microbial activity. Labeling soil-available P also allowed assessing the uptake from residual P that remained in the soils because of their different fertilization histories. On each soil, recovery of fresh manure P in four harvests of ryegrass shoots was lower than recovery of mineral P. It ranged from 24% to...
Tipo: Journal paper Palavras-chave: Nutrient turnover.
Ano: 2010 URL: http://orgprints.org/25683/1/Oberson%20et%20al%202010%20PlantSoil%20P%20fertilizers%20DOK%20soils.pdf
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Kinetics of microbial phosphorus uptake in cultivated soils Organic Eprints
Oehl, Fritz; Oberson, Astrid; Probst, Mirijam; Fliessbach, Andreas; Roth, Hans-Rudolf; Frossard, Emmanuel.
Knowledge about the role of microorganisms in P cycling at conditions of constant soil respiration rates and constant size of microbially bound P is lacking. To study the kinetics of microbial P uptake and cycling under such conditions, soils differing in biological activity were 33PO4 labelled by introducing a carrier-free tracer solution and incubated for 56 days. The 33PO4 incorporation into the fraction of microbial P releasable by chloroform treatment (Pchl) was assessed and the isotopic composition [=specific activity (SA); SA=33PO4/31PO4] of Pchl and soil solution P compared. Soils were taken from a 20-year-old field experiment including a nonfertilised control (NON), a minerally fertilised conventional (MIN) and two organic farming systems...
Tipo: Journal paper Palavras-chave: Nutrient turnover; Soil biology; Soil quality.
Ano: 2001 URL: http://orgprints.org/2934/1/oehl%2Det%2Dal%2D2000%2Dmicrobial%2Dphosphorus.pdf
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Multi-purpose cowpea inoculation for improved yields in small holder farms in Kenya Organic Eprints
Mathu, Samuel; Thonar, Cécile; Vanlauwe, Bernard; Messmer, Monika; Frossard, Emmanuel.
Introduction In Kenya, cowpea is the most important pulse crop in the dry lands of Eastern and Coastal regions where it is commonly inter cropped with maize and sorghum. The poor yields obtained in small holder farms in Kenya (150 kg ha-1) can in part be attributed to the use of poor yielding varieties, low soil fertility (mainly N and P deficiency) low presence of effective indigenous rhizobia and high occurrence of highly competitive but inefficient indigenous rhizobia strains. Biological nitrogen fixation (BNF) through exploitation of the rhizobia-legume symbiosis and use of inoculants coupled with soil amendments such as Phosphorus offers in part a means to improve cowpea yield, nutrition and soil fertility.
Tipo: Conference paper, poster, etc. Palavras-chave: Soil quality Breeding; Genetics and propagation Africa.
Ano: 2012 URL: http://orgprints.org/22169/1/mathu-etal-2012-Poster-ISFM-nairobi-kenya.pdf
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Wurzelbürtige Stickstoff- und Kohlenstoffeinträge in den Boden: Erkenntnisse aus sieben Jahren DOK Wurzelforschung Organic Eprints
Mayer, Jochen; Hirte, Juliane; Oberson, Astrid; Lüscher, Andreas; Frossard, Emmanuel; Mäder, Paul; Hammelehle, Andreas.
We investigated nitrogen and carbon below ground inputs of soybean, clover-grass and maize and management effects of biological and conventional systems in the DOK long term experiment. We found for soybean increasing below ground C and N inputs with decreasing fertilization intensity whereas those effects were small for clover-grass. Below ground nitrogen inputs of red clover could be estimated by above ground N multiplied with a factor of 0.5.
Tipo: Conference paper, poster, etc. Palavras-chave: Soil quality Cereals; Pulses and oilseeds Nutrient turnover.
Ano: 2015 URL: http://orgprints.org/27229/1/27229_mayer.pdf
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Evaluation of MALDI-TOF mass spectrometry for the competitiveness analysis of selected indigenous cowpea (Vigna unguiculata L. Walp.) Bradyrhizobium strains from Kenya Organic Eprints
Mathu Ndungu, Samuel; Messmer, Monika M.; Ziegler, Dominik; Thuita, Moses; Vanlauwe, Bernard; Frossard, Emmanuel; Thonar, Cécile.
Cowpea N2 fixation and yield can be enhanced by selecting competitive and efficient indigenous rhizobia. Strains from contrasting agro-ecologies of Kilifi and Mbeere (Kenya) were screened. Two pot experiments were established consisting of 13 Bradyrhizobium strains; experiment 1 (11 Mbeere + CBA + BK1 from Burkina Faso), experiment 2 (12 Kilifi + CBA). Symbiotic effectiveness was assessed (shoot biomass, SPAD index and N uptake). Nodule occupancy of 13 simultaneously co-inoculated strains in each experiment was analyzed by matrix-assisted laser desorption/ionization time of flight (MALDI-TOF) mass spectrometry (MS) to assess competitiveness. Strains varied in effectiveness and competitiveness. The four most efficient strains were further evaluated in a...
Tipo: Journal paper Palavras-chave: Crop health; Quality; Protection Africa.
Ano: 2018 URL: http://orgprints.org/33100/1/Ndungu-etal-2018-AMB-online-p1-14.pdf
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Microbially-mediated P fluxes in calcareous soils as a function of water-extractable phosphate Organic Eprints
Schneider, Kimberly D.; Voroney, R. Paul; Lynch, Derek H.; Oberson, Astrid; Frossard, Emmanuel; Bünemann, Else K..
Soil phosphorus (P) tests are designed to indicate plant-available inorganic orthophosphate (Pi), but fail to account for Pi that may become available through organic phosphorus (Po) mineralization. This P source may be especially important in soils with low concentrations of solution and labile Pi. We assessed gross Po mineralization and immobilization using labeling with 33P in four calcareous Alfisols with varying concentrations of Olsen soil test P that were collected from forage fields of dairy farms in Ontario, Canada. Rapid microbial 33P uptake during incubation was found for the soils with the lowest available Pi as indicated by both Olsen soil test P and water-extractable Pi. The tracer incorporation into microbial P after 8 days ranged from 7 to...
Tipo: Journal paper Palavras-chave: Soil quality; Environmental aspects.
Ano: 2017 URL: http://orgprints.org/32322/1/schneider-etal-SoilBiolBiochem-Vol106-p51-60.pdf
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Stickstoffbilanzen in biologischen und konventionellen Anbausystemen Das Effizienz-Nachhaltigkeits-Dilemma Organic Eprints
Mayer, Jochen; Jarosch, KA.; Hammelehle, Andreas; Dubois, David; Gunst, Lucie; Bosshard, C.; Frossard, Emmanuel; Mäder, Paul; Oberson, Astrid.
N-balances over 35 years from the DOK trial are presented and combined with Nstock changes in DOK treatments on different fertilisation levels. Results strongly indicate an N efficiency-sustainability dilemma: DOK treatments with a high nitrogen use efficiency (NUE) lose more soil stock N than those with a lower NUE but higher N losses from the system. The biodynamic system showed little advantage in terms of soil N stocks sustainability while the solely mineral fertilised conventional treatment had highest NUE across all inputs including soil N change.
Tipo: Conference paper, poster, etc. Palavras-chave: Biodiversity and ecosystem services; Farm nutrient management.
Ano: 2017 URL: http://orgprints.org/31925/1/Stickstoffbilanzen%20in%20biologischen%20und%20konventionellen.pdf
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Symbiotische Stickstofffixierung in biologisch und konventionell bewirtschafteten Wiesen Organic Eprints
Oberson, Astrid; Frossard, Emmanuel; Mayer, Jochen; Flura, Thomas; Mäder, Paul; Lüscher, Andreas.
Because of lower nitrogen (N) input it is assumed that organically cultivated legumes fix more N2 than legumes grown under conventional cropping. Using the natural 15N abundance method, we assessed symbiotic N2 fixation by white and red clover. The clover was growing in a grass-clover meadow installed as part of the crop rotation of a long term (30 years) field experiment. Dry matter yields were similar for organic and conventional meadows on plots fertilized at levels typical for the respective system, but organic meadows tended to higher clover yields. The proportion of N derived from atmosphere (Ndfa) in both clover species was on average 84%. It was not significantly affected by the cropping system. Because of more legumes, amounts of fixed N2...
Tipo: Conference paper, poster, etc. Palavras-chave: Pasture and forage crops.
Ano: 2009 URL: http://orgprints.org/14215/1/Oberson_14215.pdf
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Soil phoD and phoX alkaline phosphatase gene diversity responds to multiple environmental factors Organic Eprints
Ragot, Sabine A.; Kertesz, Michael A.; Mészáros, Éva; Frossard, Emmanuel; Bünemann, Else K..
Alkaline phosphatases such as PhoD and PhoX are important in organic phosphorus cycling in soil. We identified the key organisms harboring the phoD and phoX genes in soil and explored the relationships between environmental factors and the phoD- and phoX-harboring community structures across three land uses located in arid to temperate climates on two continents using 454-sequencing. phoD was investigated using recently published primers, and new primers were designed to study phoX in soil. phoD was found in 1 archaeal, 13 bacterial and 2 fungal phyla, and phoX in 1 archaeal and 16 bacterial phyla. Dominant phoD-harboring phyla were Actinobacteria, Cyanobacteria, Deinococcus-Thermus, Firmicutes, Gemmatimonadetes, Planctomycetes and Proteobacteria, while...
Tipo: Journal paper Palavras-chave: Soil quality; Environmental aspects.
Ano: 2017 URL: http://orgprints.org/32317/1/ragot-etal-2017-FEMS-Vol93-Issue1-fiw212.pdf
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Stickstoffausnutzungseffizienz von 15N-markierter Schafsgülle und 15N-markiertem Mineraldünger in biologisch und konventionell bewirtschafteten Anbausystemen Organic Eprints
Bosshard, Christine; Sorensen, Peter; Frossard, Emmanuel; Mayer, Jochen; Mäder, Paul; Nanzer, Simone; Oberson, Astrid.
Nitrogen (N) utilisation by crops has to be improved to minimize losses to the environment. We investigated N use efficiency of animal manure and mineral fertiliser and fate of fertiliser N not taken up by crops in a bio-organic (BIOORG) and a conventional (CONMIN) cropping system of a long-term experiment over three vegetation periods (wheat-soybean-maize). Microplots received a single application of 15N-labelled slurries or mineral fertiliser. At the end of each vegetation period we tested whether higher microbial activity and biomass in BIOORG than CONMIN soils and lower long-term N input level in BIOORG affected use efficiency and fate of fertiliser N not taken up by crops. In total 41%, 15% and 50% of 15N applied as urine, faeces and mineral...
Tipo: Conference paper, poster, etc. Palavras-chave: Nutrient turnover.
Ano: 2009 URL: http://orgprints.org/14392/1/Bosshard_14392.pdf
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