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Furian,TQ; Borges,KA; Pilatti,RM; Almeida,C; Nascimento,VP do; Salle,CTP; Moraes,HL de S. |
The ability of Pasteurella multocida to invade and multiply in its host is enhanced by the presence of the capsule, one of the most important virulence factors for this bacterium. Capsular typing methods are often used in epidemiological and pathogenesis studies of this agent. Five different serogroups have been identified based on serological typing. However, such tests are laborious, and agglutination of homologous antiserum may fail. The aim of this study was to develop a multiplex PCR protocol for the identification of the hyaD-hyaC and dcbF genes specific to serogroups A and D, respectively, and to compare these results with those of phenotypic tests for 54 strains isolated from fowl cholera cases in southern Brazil. The kappa coefficient and... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Molecular diagnosis; Non-serologic tests; Pasteurellosis; Serogroup. |
Ano: 2014 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2014000200004 |
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Teles,PFS; Boiago,MM; Frigo,A; Rampazzo,L; Araújo,DN; Kich,JD; Rebelatto,R; Furian,TQ; Stefani,LM. |
ABSTRACT Brazil is the largest exporter of chicken meat and poultry farming is one of the most important productive segments, despite major losses due to the bacterium Escherichia coli, which is also a zoonotic microorganism. The objetive of this study was to isolate E. coli and to evaluate its transmissibility potential from the field to chicken meat using the Pulsed Field Gel Electrophoresis (PFGE) technique. Environmental samples (poultry litter, soil and water) were collected from broiler farms located in the South of Brazil where the majority of the Brazilian poultry production occurs. In addition, chicken meat (gizzard, heart, drumette and tulip) samples were collected from local supermarkets. As results, 47.36% of the samples were positives for E.... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Environment; Epidemiology; Microbiology; Public health; Pulsed field gel electrophoresis. |
Ano: 2021 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2021000100308 |
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Camilotti,E; Moraes,LB; Furian,TQ; Borges,KA; Moraes,HLS; Salle,CTP. |
ABSTRACT The Infectious Bursal Disease (IBD) is a contagious viral disease that affects young chickens and may cause high morbidity and mortality. As the virus is very resistant to the environment, vaccination is required in case of high infection pressure. Due to variations in the virulence degree of the vaccines available to control IBD, this study aimed at evaluating the pathogenicity and immunogenicity of three types of vaccines. In total, 220 one-day-old specific pathogen free (SPF) chickens were immunized with recombinant, immune-complex and intermediate vaccines, or not vaccinated (55 birds per group) and challenged with IBD G11 strain on day 25. On days 25, 30, and 35, the Bursa of Fabricius (BF) were submitted to gross and histological... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Infectious Bursal Disease; Chickens; Vaccine; Immunity. |
Ano: 2016 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2016000200303 |
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Paravisi,M; Laviniki,V; Bassani,J; Kunert Filho,HC; Carvalho,D; Wilsmann,DE; Borges,KA; Furian,TQ; Salle,CTP; Moraes,HLS; Nascimento,VP. |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Antimicrobial resistance; Campylobacter jejuni; GyrA; Mutation; Poultry; TetO. |
Ano: 2020 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2020000200323 |
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Moraes,HLS; Salle,CTP; Nascimento,VP; Salle,FO; Rocha,ACGP; Souza,GF; Furian,TQ; Artencio,JO. |
Broiler chicks belonging to two poultry companies, A and B, with different breeders' vaccination programs were challenged with a very virulent strains of infectious bursal disease virus (vvIBDV), genotyped as G11. Birds were separated in four groups, two vaccinated at the first day of life and two unvaccinated. They were then challenged at the 1st, 4th, 7th, 10th, 13th, 16th, 19th and 22nd days. At every day of challenge, before and after the procedure, the following data were collected from each group: Bursa of Fabricius (BF) relative weight, BF diameter, BF for histologie examination, serum for measuring antibodies against IBDV through the ELISA and clinical evaluation of IBD. The results obtained have shown a non-significant drop in antibody level... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Infectious bursal disease; Very very virulent IBD virus; Vaccination; Maternal immunity. |
Ano: 2005 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2005000100009 |
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Mandelli,JZA; Ehrhardt,A; Manto,L; Borges,KA; Furian,TQ; Weber,B; Rodrigues,LB; Santos,LR. |
ABSTRACT Antimicrobial resistance is a serious public health problem and Salmonella spp. is highly resistant to antimicrobial agents. Biofilms are important in the food industry due to their formation on products, utensils, and surfaces and the difficulty in their removal. The objective of this study was to assess extended spectrum beta-lactamase (ESBL) production, antimicrobial resistance, and biofilm production of Salmonella isolated from poultry slaughterhouses. Antimicrobial susceptibility was assessed by the disk diffusion assay and ESBL by double diffusion disk assay using the beta-lactamase inhibitor (amoxicillin+clavulanate). The antimicrobials tested were: ampicillin, amoxicillin+clavulanate, aztreonam, ceftazidime, cefotaxime, chloramphenicol,... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Biofilms; Drug resistance; ESBL; Salmonella. |
Ano: 2019 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2019000200348 |
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Pilatti,RM; Furian,TQ; Lima,DA; Finkler,F; Brito,BG; Salle,CTP; Moraes,HLS. |
ABSTRACT Although Pasteurella multocida is a member of the respiratory microbiota, under some circumstances, it is a primary agent of diseases , such as fowl cholera (FC), that cause significant economic losses. Experimental inoculations can be employed to evaluate the pathogenicity of strains, but the results are usually subjective and knowledge on the pathogenesis of this agent is still limited. The objective of this study was to establish a new methodology for classifying the pathogenicity of P. multocida by formulating a standard index. Strains isolated from FC cases and from swine with respiratory problems were selected. One hundred mL of a bacterial culture of each strain, containing 106 CFU, was inoculated in 10 one-day-old broilers. Mortality after... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Fowl cholera; Inoculation; Pasteurella multocida; Pathogenicity index. |
Ano: 2016 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2016000200255 |
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