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	Provedor de dados Rev. Bras. Ciênc. Avic. (1) Autor Brentano,L (1) Furuyama,CRAG (1) Givisiez,PEN (1) Montassier,HJ (1) Montassier,MFSM (1) Okino,CH (1) Mais... Palavra-chave Detection (1) Differentiation (1) Infectious bronchitis virus (1) Semi-nested RT-PCR (1) Vaccine strain (1) Tipo do documento Info:eu-repo/semantics/article (1) Ano 2005 (1) País Brazil (1) Idioma Inglês (1)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 1 Primeira ... 1 ... Última Infectious bronchitis virus: detection and vaccine Strain differentiation by semi-nested RT-PCR Rev. Bras. Ciênc. Avic. Okino,CH; Montassier,MFSM; Givisiez,PEN; Furuyama,CRAG; Brentano,L; Montassier,HJ. A semi-nested reverse transcription-polymerase chain reaction (Semi-N-RT-PCR) was developed and used to detect the S glycoprotein gene of infectious bronchitis virus (IBV) strains and to discriminate H120 vaccine strain from other strains. Viral RNA was extracted from the allantoic fluid of chicken embryos and from tissues of chickens experimentally infected with different strains of IBV. Amplification and identification of the viral RNA was performed using two sets of primers complementary to a region of the S glycoprotein gene in the Semi-N-RT-PCR assay. The pair of primers used in the first PCR consisted of universal oligonucleotides flanking a more variable region of S1-S2 gene. The second primer pair was used in the Semi-N-RT-PCR and was comprised of... Tipo: Info:eu-repo/semantics/article Palavras-chave: Detection; Differentiation; Infectious bronchitis virus; Semi-nested RT-PCR; Vaccine strain. Ano: 2005 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2005000100010 Registros recuperados: 1 Primeira ... 1 ... Última

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