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Optimization of biomass, total carotenoids and astaxanthin production in Haematococcus pluvialis Flotow strain Steptoe (Nevada, USA) under laboratory conditions Biol. Res.
CIFUENTES,ANA S; GONZÁLEZ,MARIELA A; VARGAS,SILVIA; HOENEISEN,MARITZA; GONZÁLEZ,NELSON.
The microalga Haematococcus pluvialis Flotow is one of the natural sources of astaxanthin, a pigment widely used in salmon feed. This study was made to discover optimal conditions for biomass and astaxanthin production in H. pluvialis from Steptoe, Nevada (USA), cultured in batch mode. Growth was carried out under autotrophic (with NaNO3, NH4Cl and urea) and mixotrophic conditions (with 4, 8, 12 mM sodium acetate) under two photon flux densities (PFD) (35 and 85 µmol m-2 s-1). The carotenogenesis was induced by 1) addition of NaCl (0.2 and 0.8 %), 2) N-deprivation and 3) high PFD (150 µmol m-2 s-1). Total carotenoids were estimated by spectrophotometry and total astaxanthin by HPLC. Ammonium chloride was the best N-source for growth (k=0.7 div day-1,...
Tipo: Journal article Palavras-chave: Astaxanthin; Carotenogenesis; Growth; Haematococcus pluvialis.
Ano: 2003 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602003000300006
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