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| POMPEU,Dávia Guimarães; MATTIOLI,Marcelo Augusto; RIBEIRO,Rosy Iara Maciel de Azambuja; GONÇALVES,Daniel Bonoto; MAGALHÃES,Juliana Teixeira de; MARANGONI,Sérgio; SILVA,José Antônio da; GRANJEIRO,Paulo Afonso. |
| Abstract A novel lectin was isolated from the seeds of Chenopodium quinoa. To achieve this end, the crude extract from the quinoa was submitted to two purification steps, Sephadex G50 and Mono Q. The hemagglutinating activity showed that this lectin agglutinates human erythrocytes. Its activity is inhibited by glucose and mannose, and remained stable under a wide range of pH levels and temperatures. The quinoa lectin was found to be a heterodimeric lectin of approximately 60 kDa, consisting of two subunits of approximately 25 kDa and 35 kDa. This lectin had its antimicrobial activity tested against several bacteria strains and effectively inhibited three strains. These strains were all Gram-negative, making this lectin a promising antimicrobial tool. |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Antimicrobial; Chenopodium quinoa; Glucose/mannose-specific; Lectin; Seeds. |
| Ano: 2015 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612015000400696 |
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