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| Vaulot, Daniel; Lepere, Cecile; Toulza, Eve; De La Iglesia, Rodrigo; Poulain, Julie; Gaboyer, Frederic; Moreau, Herve; Vandepoele, Klaas; Ulloa, Osvaldo; Gavory, Frederick; Piganeau, Gwenael. |
| Among small photosynthetic eukaryotes that play a key role in oceanic food webs, picoplanktonic Mamiellophyceae such as Bathycoccus, Micromonas, and Ostreococcus are particularly important in coastal regions. By using a combination of cell sorting by flow cytometry, whole genome amplification (WGA), and 454 pyrosequencing, we obtained metagenomic data for two natural picophytoplankton populations from the coastal upwelling waters off central Chile. About 60% of the reads of each sample could be mapped to the genome of Bathycoccus strain from the Mediterranean Sea (RCC1105), representing a total of 9 Mbp (sample T142) and 13 Mbp (sample T149) of non-redundant Bathycoccus genome sequences. WGA did not amplify all regions uniformly, resulting in unequal... |
| Tipo: Text |
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| Ano: 2012 |
URL: http://archimer.ifremer.fr/doc/00129/23987/21945.pdf |
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| Ciobanu, Maria Cristina; Burgaud, Gaetan; Dufresne, Alexis; Breuker, Anja; Redou, Vanessa; Ben Maamar, Sarah; Gaboyer, Frederic; Vandenabeele-trambouze, Odile; Lipp, Julius Sebastian; Schippers, Axel; Vandenkoornhuyse, Philippe; Barbier, Georges; Jebbar, Mohamed; Godfroy, Anne; Alain, Karine. |
| The subsurface realm is colonized by microbial communities to depths of >1000 meters below the seafloor (m.b.sf.), but little is known about overall diversity and microbial distribution patterns at the most profound depths. Here we show that not only Bacteria and Archaea but also Eukarya occur at record depths in the subseafloor of the Canterbury Basin. Shifts in microbial community composition along a core of nearly 2 km reflect vertical taxa zonation influenced by sediment depth. Representatives of some microbial taxa were also cultivated using methods mimicking in situ conditions. These results suggest that diverse microorganisms persist down to 1922 m.b.sf. in the seafloor of the Canterbury Basin and extend the previously known depth limits of... |
| Tipo: Text |
Palavras-chave: Deep biosphere; Subsurface life; Eukaryote; Record depth. |
| Ano: 2014 |
URL: http://archimer.ifremer.fr/doc/00201/31243/29895.pdf |
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| Gaboyer, Frederic; Tindall, Brian J.; Ciobanu, Maria Cristina; Duthoit, Frederique; Le Romancer, Marc; Alain, Karine. |
| A novel Gram-stain-negative, strictly aerobic, heterotrophic bacterium, designated 306(T), was isolated from near-surface (109 cm below the sea floor) sediments of the Gulf of Lions, in the Mediterranean Sea. Strain 306(T) grew at temperatures between 4 and 32 degrees C (optimum 17-22 degrees C), from pH 6.5 to 9.0 (optimum 8.0-9.0) and between 0.5 and 6.0% (w/v) NaCl (optimum 2.0%). Its DNA G+C content was 58.8 mol%. On the basis of 16S rRNA gene sequence similarity, the novel isolate belongs to the class Alphaproteobacteria and is related to the genus Phaeobacter. It shares 98.7% 16S rRNA sequence identity with Phaeobacter arcticus, its closest phylogenetic relative. It contained Q-10 as the only respiratory quinone, C-18:1 omega 7c and C-16:0 as major... |
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| Ano: 2013 |
URL: http://archimer.ifremer.fr/doc/00166/27750/25981.pdf |
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| Gaboyer, Frederic; Vandenabeele-trambouze, Odile; Cao, Junwei; Ciobanu, Maria-cristina; Jebbar, Mohamed; Le Romancer, Marc; Alain, Karine. |
| A novel halophilic bacterium, strain RHS90T, was isolated from marine sediments from the Gulf of Lions, in the Mediterranean Sea. Its metabolic and physiological characteristics were examined under various cultural conditions, including exposure to stressful ones (oligotrophy, high pressure and high concentrations of metals). Based on phylogenetic analysis of the 16S rRNA gene, the strain was found to belong to the genus Halomonas in the class Gammaproteobacteria. Its closest relatives are H. axialensis and H. meridiana (98% similarity). DNA-DNA hybridizations indicated that the novel isolate is genotypically distinct from these species. The DNA G+C content of the strain is 54.4 mol%. The main fatty acids (C18:1ω7c, 2-OH iso-C15:0, C16:0 and/or C19:0 cyclo... |
| Tipo: Text |
Palavras-chave: Halomonas; Taxonomy; Environmental adaptation; Metal tolerance; Poly-beta-hydroxyalkanoate; Compatible. |
| Ano: 2014 |
URL: http://archimer.ifremer.fr/doc/00201/31253/29658.pdf |
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