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| Gagne, N; Cochennec, Nathalie; Stephenson, M; Mcgladdery, S; Meyer, G; Bower, S. |
| As part of a disease resistance experiment, 112 apparently healthy European flat oysters Ostrea edulis L. were exported from Canada (Nova Scotia) into France to test their susceptibility to Bonamia ostreae infection. Twelve oysters died in transit and 17 others died within 2 wk of laboratory quarantine acclimation. All oysters were examined histologically, and the 17 that died during quarantine were assayed for microcells (Bonamia sp. and Mikrocytos mackini) using molecular techniques. A microcell parasite was detected in the connective tissue of 5 of the 112 oysters. Morphological appearance, tissue affinity and molecular characterization through PCR, in situ hybridization (ISH), fluorescence in situ hybridization (FISH) and sequencing revealed a protist... |
| Tipo: Text |
Palavras-chave: Histology; PCR; In situ hybridization; Ostrea edulis; Mikrocytos. |
| Ano: 2008 |
URL: http://archimer.ifremer.fr/doc/2008/publication-4475.pdf |
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