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Sex-Specific Regulation of AMP-Activated Protein Kinase (AMPK) in the Pacific Oyster Crassostrea gigas ArchiMer
Guevelou, Eric; Huvet, Arnaud; Galindo-sanchez, Clara E.; Milan, Massimo; Quillien, Virgile; Daniel, Jean-yves; Quere, Claudie; Boudry, Pierre; Corporeau, Charlotte.
The hermaphrodite Pacific oyster Crassostrea gigas displays a high energy allocation to reproduction. We studied the expression of AMP-activated protein kinase (AMPK) during gametogenesis in the gonad and characterized the mRNA sequences of the AMPK subunits: the AMPK alpha mRNA sequence was previously characterized; we identified AMPK beta, AMPK gamma, and mRNAs of putative AMPK-related targets following bioinformatics mining on existing genomic resources. We analyzed the mRNA expression of the AMPK alpha, beta, and gamma subunits in the gonads of male and female oysters through a reproductive cycle, and we quantified the mRNA expression of genes belonging to fatty acid and glucose metabolism. AMPK alpha mRNA levels were more abundant in males at the...
Tipo: Text Palavras-chave: AMP-activated protein kinase; Crassostrea gigas; Gametogenesis; Marine bivalve; Reproduction.
Ano: 2013 URL: http://archimer.ifremer.fr/doc/00168/27911/26470.pdf
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