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Towards genetic transformation of local onion varieties Electron. J. Biotechnol.
Marinangeli,Pablo; Deluchi,Bernardo; Ríos,Raúl; Franzone,Pascual; Galmarini,Claudio; Rosselló,Fernando; Curvetto,Néstor.
The aim of this work was to explore the possibility of obtaining transgenic plants of onion varieties cultivated in Argentina, starting from calli induced from mature zygotic embryos, using two strains of Agrobacterium tumefaciens as transfection vectors. Mature embryos from three varieties of 'Valenciana' onion, Torrentina, Cobriza INTA and Grano de oro were in vitro cultivated for callus induction. After three to four months an average of 57.4% success for the three varieties was reached. Transformation was carried out with Agl1 or LBA 4404 A. tumefaciens strains, both carrying a binary vector containing the marker gene gus a and the selection gene nptII. Selection was done in callus induction medium containing 10 mgL-1 geneticin during three...
Tipo: Journal article Palavras-chave: Agrobacterium tumefaciens; Allium cepa L.; Callus; Zygotic embryos.
Ano: 2006 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582006000300030
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New insight into how thigmomorphogenesis affects Epipremnum aureum plant development Horticultura Brasileira
Benedetto,Adalberto Di; Galmarini,Claudio; Tognetti,Jorge.
ABSTRACT Climbing Epipremnum aureum plants develop larger leaves than unsupported, hanging plants. This effect may be regarded, in part, as a thigmomorphogenic response, but gravimorphogenetic effect may also be involved, since polar auxin transport is known to be negatively affected in plants with horizontal or hanging stems, which may result in an altered hormone balance at the whole plant level. The present work was aimed at studying how exogenous auxins and cytokinins may influence growth of E. aureum rooted cuttings under different training systems. Rooted cuttings of E. aureum were cultivated either climbing on an upright wooden board or creeping on the glasshouse bench or hanging from a basket. All leaves of each plant were sprayed to run-off at...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Auxin; Citokinin; Foliage plants; Gravitropism; Leaf growth; Leaf anatomy; Photosynthesis.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-05362018000300330
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