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Production of Rhodotorula glutinis: a yeast that secretes alpha-L-arabinofuranosidase Electron. J. Biotechnol.
Martínez,Claudio; Gertosio,Cecilia; Labbe,Angelica; Pérez,Rafael; Ganga,Maria Angélica.
Rhodotorula glutinis is a yeast that secretes the enzyme alpha-L-arabinofuranosidase (E.C. 3.2.1.55) into the culture medium and thus has an interesting biotechnological potential. To determine improved culture conditions of this organism, different factors of the culture media were evaluated such as the use of peptone as nitrogen source, salts composition, pH and growth temperature. Likewise, beet molasses and beet cosette were tested as industrial carbon sources to induce the production of the enzyme and how they influence the yeast growth. Based on these studies a culture medium is proposed for growth of this yeast in a continuous system. By assaying different dilution rates an average specific activity for the enzyme of 82.4 U/mg of protein was obtained.
Tipo: Journal article Palavras-chave: Alpha-L-arabinofuranosidase yeast; Enzymes; Wine.
Ano: 2006 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582006000400009
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Gene expression of specific enological traits in wine fermentation Electron. J. Biotechnol.
Martínez,Claudio; García,Verónica; González,David; Jara,Matías; Aguilera,Matías; Ganga,Maria Angélica.
Background: Wine fermentation is a dynamic process and yeast has a precise genic regulation system that allow them to coordinate the gene transcription levels. However, the fermentation stage at which there is a great variation in the genic expression associated to a particular enological trait is often unknown and there are no simple experimental approaches to define it. Results: To identify the most adequate stage in which to evaluate the expression of the genes associated to specific enological traits we identified three stages of fermentation using the industrial strain EC1118. These stages, called early (0-16.4 hrs), middle (16.4-88.7 hrs) and late (88.7 hrs onwards), were characterized according to changes in the speed of CO2 production. The greatest...
Tipo: Journal article Palavras-chave: Enological traits; Gene expression; Saccharomyces cerevisiae; Wine fermentation; Wine yeast.
Ano: 2013 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582013000400013
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