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Preliminary evaluation of adherence on abiotic and cellular surfaces of Acinetobacter baumannii strains isolated from catheter tips BJID
Costa,Gislaine Franco de Moura; Tognim,Maria Cristina Bronharo; Cardoso,Celso Luíz; Carrara-Marrone,Floristher Elaine; Garcia,Lourdes Botelho.
The cell surface hydrophobicity and adhesion to abiotic and cellular surfaces was tested in five clinical strains of Acinetobacter baumannii isolated from catheter tips. Biochemical and molecular characteristics of these strains were also studied. Hydrophobicity was characterized by a test for affinity to xylene. Adhesion to abiotic surfaces (polystyrene, formica, latex and glass) was evaluated in Petri plates using the stamp technique. Buccal epithelial cells were used for tests of adhesion to cellular surfaces. Adhesion to the catheter was evaluated by repeatedly rinsing the catheters and rolling them over nutrient agar. Molecular typing of the strains was done by the ERIC-PCR technique. The degree of hydrophobicity of the strains varied from hydrophobic...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Acinetobacter baumannii; Hydrophobicity; Adherence.
Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702006000500009
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Nasal carriage of methicillin-resistant Staphylococcus aureus in university students BJID
Prates,Karina Aparecida; Torres,Ana Maria; Garcia,Lourdes Botelho; Ogatta,Sueli Fumie Yamada; Cardoso,Celso Luiz; Tognim,Maria Cristina Bronharo.
In a study of university students, the percentage nasal carriage of Staphylococcus aureus was 40.8% (102/250). Of the isolates, MIC50 of methicillin was 0.5 µg/mL and MIC90 was 1 µg/mL. Six (5.8%) isolates were methicillin-resistant and carried the mecA gene. These results suggest that community-associated methicillin-resistant S. aureus may be spreading in Brazil.
Tipo: Info:eu-repo/semantics/other Palavras-chave: Nasal carriage; CA-MRSA; S. aureus.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702010000300021
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Disinfection of gutta-percha cones with chlorhexidine BJM
Redmerski,Roberta; Bulla,Joice Renata; Moreno,Tatiana; Garcia,Lourdes Botelho; Cardoso,Celso Luiz.
This study investigated the effectiveness of detergent and aqueous solutions of 2% chlorhexidine digluconate in decontaminating gutta-percha cones (gpc) contaminated with bacteria, yeast, or bacterial spores. Gutta-percha cones were contaminated with 10(7)-10(8) colony-forming units per milliliter (cfu/ml) of the following test organisms: Staphylococcus aureus, Enterococcus faecalis, Escherichia coli, or Candida albicans. Spores of Bacillus subtilis were also tested. Contaminated gpc were treated with the chlorhexidine solutions for 1, 5, 10, or 15 min. Each cone was then transferred to a tube containing saline and the micoorganisms were recovered after homogenization for cfu determination. Both detergent and aqueous chlorhexidine solutions were effective...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Chlorhexidine; Decontamination; Gutta-percha cones.
Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822007000400013
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The effectiveness of alcohol gel and other hand-cleansing agents against important nosocomial pathogens BJM
Hernandes,Silvio Evandro Daniel; Mello,Aline Cláudia de; Sant'Ana,Janete Jordão; Soares,Vanessa Sarto; Cassiolato,Valdir; Garcia,Lourdes Botelho; Cardoso,Celso Luíz.
We compared the effectiveness of alcohol gel with that of the traditional hand-cleansing agents in removing clinical strains of Acinetobacter baumannii, methicillin-resistant Staphylococcus aureus, Escherichia coli, Enterococcus faecalis, Pseudomonas aeruginosa, and Candida albicans from artificially contaminated hands. The fingertips of 6 volunteers were contaminated with approximately 10(6) of microbial cells, and then were washed with: plain liquid soap, alcohol gel, 70% ethyl alcohol (by weight), 10% povidone-iodine liquid soap (PVP-I), and 4% chlorhexidine gluconate detergent. The experiments were performed using a Latin square statistical design, with six 6 x 5 randomized blocks, and the results were estimated by ANOVA. The products reduced from...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Alcohol gel; Hand-cleansing agents; Nosocomial pathogens.
Ano: 2004 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822004000100005
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Stpahylococcus aureus biofilms on central venous haemodialysis catheters BJM
Aoki,Elisabeth Eyko; Pizzolitto,Antonio Carlos; Garcia,Lourdes Botelho; Pizzolitto,Elisabeth Loshchagin.
Biofilm bacterial infections are common in patients undergoing treatment with haemodialysis. This study involved 16 patients (7 males, 9 females; ages from 22 to 81 with an average age of 50) who had had a total of 25 temporary haemodialysis polyurethane catheter insertions into the subclavian vein (22 dual-lumen and 3 triple-lumen). The catheters remained in place from 3 to 91 days, on an average of 47 days. The reasons for catheter removal were: bad functioning (44%), suspicion of catheter-related infection (20%), availability of permanent access (16%), accidental removal (12%), signs and symptoms of infection at the site of catheter insertion (4%), and exogenous contamination (4%). Positive tip cultures were observed on seven of the catheters (28%),...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Biofilm; Catheter in haemodialysis; Central venous catheter; Catheter-related infections.
Ano: 2005 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822005000400007
Registros recuperados: 5
Primeira ... 1 ... Última
 

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