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| Degrandi,Tiago Marafiga; Pita,Sebastian; Panzera,Yanina; Oliveira,Edivaldo Herculano C. de; Marques,José Ribamar Felipe; Figueiró,Marivaldo Rodrigues; Marques,Larissa Coêlho; Vinadé,Lucia; Gunski,Ricardo José; Garnero,Analía Del Valle. |
| Domestic buffaloes are divided into two group based on cytogenetic characteristics and habitats: the "river buffaloes" with 2n = 50 and the "swamp buffaloes", 2n = 48. Nevertheless, their hybrids are viable, fertile and identified by a 2n = 49. In order to have a better characterization of these different cytotypes of buffaloes, and considering that NOR-bearing chromosomes are involved in the rearrangements responsible for the karyotypic differences, we applied silver staining (Ag-NOR) and performed fluorescent in situ hybridization (FISH) experiments using 18S rDNA as probe. Metaphases were obtained through blood lymphocyte culture of 21 individuals, including river, swamp and hybrid cytotypes. Ag-NOR staining revealed active NORs on six chromosome pairs... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: 18S rDNA; Bubalus bubalis; FISH. |
| Ano: 2014 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572014000300009 |
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| Souza,Marcelo Santos de; Barcellos,Suziane Alves; Costa,Alice Lemos; Kretschmer,Rafael; Garnero,Analía Del Valle; Gunski,Ricardo José. |
| Abstract The Passeriformes is the most diverse and cytogenetically well-known clade of birds, comprising approximately 5,000 species. The sooty-fronted spinetail (Synallaxis frontalis Aves: Furnariidae) species, which belongs to the order Passeriformes, is typically found in South America, where it is widely distributed. Polymorphisms provide genetic variability, important for several evolutionary processes, including speciation and adaptation to the environment. The aim of this work was to analyze the possible cytotypes and systemic events involved in the species polymorphism. Of the sampled 19 individuals, two thirds were polymorphic, an event supposedly linked to mutations resulting from genomic evolution that can be transmitted hereditarily. A... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Chromosomal polymorphism; Evolution; Cytogenetics. |
| Ano: 2019 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572019000100062 |
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| Gunski,Ricardo José; Cunha,Isabel Souza; Degrandi,Tiago Marafiga; Ledesma,Mario; Garnero,Analía Del Valle. |
| Podocnemis expansa and P. unifilis present 2n = 28 chromosomes, a diploid number similar to those observed in other species of the genus. The aim of this study was to characterize these two species using conventional staining and differential CBG-, GTG and Ag-NOR banding. We analyzed specimens of P. expansa and P. unifilis from the state of Tocantins (Brazil), in which we found a 2n = 28 and karyotypes differing in the morphology of the 13th pair, which was submetacentric in P. expansa and telocentric in P. unifilis. The CBG-banding patterns revealed a heterochromatic block in the short arm of pair 13 of P. expansa and an interstitial one in pair 13 of P. unifilis, suggesting a pericentric inversion. Pair 14 of P. unifilis showed an insterstitial band in... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Chromosome banding; Ag-NORs; Karyotype; Chelonids. |
| Ano: 2013 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572013000300009 |
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