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Marine water quality assessment using transplanted oyster larvae ArchiMer
Quiniou, Francoise; Damiens, G; Gnassia Barelli, M; Geffard, A; Mouneyrac, C; Budzinski, H; Romeo, Michèle.
Active bio-monitoring in terms of biornarkers was attempted using Crassostrea gigas larvae produced in the laboratory and transplanted using special containers to two sites at the entrance (A) and inner part (P) of the harbour of Arcachon (French Atlantic Coast). The larvae were kept in the medium for 48 h. Their physiological status and their biomarker levels : acetylcholinesterase AChE, catalase CAT and glutathione S-transferase GST activities were determined together with metallothionein Wand Thiobarbituric Acid Reactive Substances TBARS concentrations. Copper and PAH (polycyclic aromatic hydrocarbon) concentrations were determined in the exposed larvae and in the sediments collected under the containers. Cadmium, lead and zinc could be also analyzed in...
Tipo: Text Palavras-chave: PAHs; Trace metals; Biomarkers; Active bio monitoring; Crassostrea gigas larvae.
Ano: 2007 URL: http://archimer.ifremer.fr/doc/2007/publication-2269.pdf
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Relationships between metal bioaccumulation and metallothionein levels in larvae of Mytilus galloprovincialis exposed to contaminated estuarine sediment elutriate ArchiMer
Geffard, A; Geffard, Olivier; His, Edouard; Amiard, Jc.
The bioavailability of Cd, Cu and Zn from a metal-contaminated sediment was studied using Mytilus galloprovincialis embryos and larvae exposed to sediment elutriates, The elutriate concentrations tested were always lower than the threshold at which abnormal larval development appears. The Cd, Cu and Zn contents of the sediment, of the elutriate and of the larvae, as well as the larval growth, the condition index (CI), and the induction of metallothionein (NIT) in the larvae were measured. The Cl was only affected after 96 h of exposure, principally at the highest elutriate concentration (25%, corresponding to 0.1435 mug Cd l(-1) and 14 mug Zn l(-1)). Cd, Cu and Zn bioaccumulation was observed (48 and 96 h), in whole larvae as well as in the cytosolic...
Tipo: Text Palavras-chave: Sediment elutriate; Mytilus galloprovincialis larvae; Metal bioaccumulation; Metallothionein.
Ano: 2002 URL: http://archimer.ifremer.fr/doc/00000/10119/7598.pdf
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Bioaccumulation of metals in sediment elutriates and their effects on growth, condition index, and metallothionein contents in oyster larvae ArchiMer
Geffard, A; Geffard, O; Amiard, J; His, Edouard; Amiard Triquet, C.
The bioavailability of Cd, Cu, Zn, and Pb from two metal-contaminated sediments (Bidassoa and Dunkerque) was studied using Crassostrea gigas larvae exposed to sediment elutriates. The metal contents within the sediments, the larvae and larval growth, the condition index, and the induction of metallothionein in the larvae were measured. The larval growth and condition index were only affected after exposure to the highest elutriates concentration (5 to 25%) from the most contaminated sediment (Dunkerque). Bioaccumulation of all metals was observed in larvae exposed to Dunkerque elutriatre; only Cu bioaccumulation was observed in the Bidassoa elutriate. The results from larvae exposed to both sediment elutriates show a strong correlation between...
Tipo: Text Palavras-chave: Metallothionein; Metal bioaccumulation; Larval growth; Crassostrea gigas larvae; Sediment elutriates.
Ano: 2007 URL: http://archimer.ifremer.fr/doc/2007/publication-4748.pdf
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