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| Franco,L.H.; Paula,M. Oliveira e; Wowk,P.F.; Fonseca,D.M. da; Sérgio,C.A.; Fedatto,P.F.; Gembre,A.F.; Ramos,S.G.; Silva,C.L.; Medeiros,A.I.; Faccioli,L.H.; Bonato,V.L.D.. |
| Leukotrienes are reported to be potent proinflammatory mediators that play a role in the development of several inflammatory diseases such as asthma, rheumatoid arthritis and periodontal disease. Leukotrienes have also been associated with protection against infectious diseases. However, the role of leukotrienes in Mycobacterium tuberculosis infection is not understood. To answer this question, we studied the role of leukotrienes in the protective immune response conferred by prime-boost heterologous immunization against tuberculosis. We immunized BALB/c mice (4-11/group) with subcutaneous BCG vaccine (1 x 10(5) M. bovis BCG) (prime) followed by intramuscular DNA-HSP65 vaccine (100 µg) (boost). During the 30 days following the challenge, the animals were... |
| Tipo: Info:eu-repo/semantics/other |
Palavras-chave: Tuberculosis; Prime-boost immunization; Leukotrienes. |
| Ano: 2010 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2010000700006 |
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| Rocha,C.D.; Trombone,A.P.F.; Lorenzi,J.C.C.; Almeida,L.P.; Gembre,A.F.; Padilha,E.; Ramos,S.G.; Silva,C.L.; Coelho-Castelo,A.A.M.. |
| In the last several years, the use of dendritic cells has been studied as a therapeutic strategy against tumors. Dendritic cells can be pulsed with peptides or full-length protein, or they can be transfected with DNA or RNA. However, comparative studies suggest that transfecting dendritic cells with messenger RNA (mRNA) is superior to other antigen-loading techniques in generating immunocompetent dendritic cells. In the present study, we evaluated a new therapeutic strategy to fight tuberculosis using dendritic cells and macrophages transfected with Hsp65 mRNA. First, we demonstrated that antigen-presenting cells transfected with Hsp65 mRNA exhibit a higher level of expression of co-stimulatory molecules, suggesting that Hsp65 mRNA has immunostimulatory... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Tuberculosis; Therapeutic immunization; CD; MHSP65; DNA vaccine; APCs. |
| Ano: 2012 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2012001200011 |
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