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	Provedor de dados Electron. J. Biotechnol. (2) Autor Ghosh,Purnendu (2) Kumar Nigam,Vinod (2) Kumar Gothwal,Raj (1) Kumar,Abhishek (1) Kundu,Subir (1) Mohan,M. Krishna (1) Sasmal,Dinakar (1) Verma,Ruchi (1) Mais... Palavra-chave Acremonium chrysogenum (1) Arid soils (1) Batch fermentation (1) Cephalosporin-C (1) Methionine (1) Microbial community (1) PCR-DGGE (1) Rhizosphere (1) Soil DNA extraction (1) Stirred tank bioreactor (1) Mais... Tipo do documento Journal article (2) Ano 2007 (2) País Chile (2) Idioma Inglês (2)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 2 Primeira ... 1 ... Última Extraction of bulk DNA from Thar Desert soils for optimization of PCR-DGGE based microbial community analysis Electron. J. Biotechnol. Kumar Gothwal,Raj; Kumar Nigam,Vinod; Mohan,M. Krishna; Sasmal,Dinakar; Ghosh,Purnendu. A reliable method for characterizing microbial communities on the basis of their differences in the 16S ribosomal RNA (rRNA) gene sequences in the hot arid zone sandy soils has been optimized. A desert plant (Calligonum polygonoides) was chosen to provide the rhizospheric soil samples, collected from three different agro-ecological locations. Total community DNA was efficiently extracted at small-scale level using direct lysis with hot sodium dodecyl sulphate (SDS), glass bead beating and finally subjecting the sandy soil to liquid nitrogen freeze-thaw cycles. To amplify V3 region of bacterial 16S rRNA gene, universal conserved primers were used. Second round polymerase chain reaction (PCR) was attempted to increase product concentration and to minimize... Tipo: Journal article Palavras-chave: Arid soils; Microbial community; PCR-DGGE; Rhizosphere; Soil DNA extraction. Ano: 2007 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582007000300007 Influence of medium constituents on the biosynthesis of cephalosporin-C Electron. J. Biotechnol. Kumar Nigam,Vinod; Verma,Ruchi; Kumar,Abhishek; Kundu,Subir; Ghosh,Purnendu. Acremonium chrysogenum NCIM 1069 was used for the biosynthesis of cephalosporin-C (CPC) in batch mode of cultivation. The effect of different medium constituents for better yield of CPC was thoroughly investigated. From the results of the fermentation, it was found that ammonium sulphate as inorganic nitrogen source and methionine at the concentration of 0.4% are most suitable for higher yield of antibiotic. The variation in the C/N ratio on the biosynthesis of CPC showed that a C/N ratio of 8.0 is most suitable for maximum production of CPC Tipo: Journal article Palavras-chave: Acremonium chrysogenum; Batch fermentation; Cephalosporin-C; Methionine; Stirred tank bioreactor. Ano: 2007 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582007000200007 Registros recuperados: 2 Primeira ... 1 ... Última

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