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Efeito da Mannheimia granulomatis sobre cultivo de fibroblastos Arq. Bras. Med. Vet. Zootec.
Ladeira,S.; Gomes,F.R.; Vidor,T.; Portianski,E.L.; Gimeno,E.J..
Primary cultures of Mannheimia granulomatis were established in chicken embryos to assess their capacity to stimulate fibroblast proliferation. The capacity of the bacterium-activated macrophages to stimulate cytokine and enzyme proliferation was assessed in a mouse peritoneum macrophage culture. To evaluate the bacteria infection on fibroblasts and their growth within 48h in relation to the active macrophages, cultures were washed and trypsinized and the cells counted. Results showed no significant differences when the bacteria-infected fibroblasts were mixed with bacterial extract (P=0.9682). The treatment using just products of macrophages resulted similar to the negative control. Significant differences on cell proliferation were established (P=0,0039)...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Mannheimia granulomatis; Fibroblastos; Macrófagos.
Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352008000200038
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Effects of high molybdenum intake on 1,2-dimethylhydrazine-induced intestinal tumors in rats Biocell
Montenegro,M.A.; Sánchez Negrette,M.; Gimeno,E.J.; Borda,J.T..
Wistar male rats, 3 months of age were given ad-libitum a nutritionally adequate diet and demineralized drinking water. The Molybdenum (Mo) and Tungsten (W) were provided in the drinking water at 200 ppm concentration. Intestinal tumors were induced by 1,2-dimethylhydrazine (DMH) given subcuta-neously as 16 weekly doses at 20 mg/kg body weight. Mo in the form of (NH 4 ) 6 Mo 7 O 24 4H 2 O or W in the form of (Na 2 WO 4 ) were provided in the drinking water two months before the first DMH treatment and were continued during 4 months more until the last DMH treatment. Three months after the last carcinogen injec-tion, all animals were sacrificed and examined for intestinal tumors. The number, size and location of the tumors were recorded and the pathology...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Intestinal cancer Molybdenum 1; 2-dimethylhydrazine Rats.
Ano: 2002 URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452002000300004
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