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	Provedor de dados 69 (1) Autor Giordano,Oscar S (1) Hernandez,Xenia E (1) Kurina,Marcela (1) Orden,Alejandro A (1) Palavra-chave Cu2+ (1) Dimethylsulfoxide permeabilization (1) Grindelane diterpenes (1) Jasmonic acid (1) Tipo do documento Journal article (1) Ano 2005 (1) País Chile (1) Idioma Inglês (1)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 1 Primeira ... 1 ... Última Effects of elicitor and copper sulfate on grindelic acid production in submerged cultures of Grindelia pulchella 69 Hernandez,Xenia E; Orden,Alejandro A; Giordano,Oscar S; Kurina,Marcela. Grindelia pulchella callus and cell suspension cultures were established from seedling leaves. When several phytoregulator supplementations were assayed in solid Murashige and Skoog medium containing 3% (w/v) of sucrose (MS medium), combinations of indole-3-butyric acid (IBA) and N6-benzylaminopurine (BA) resulted the most appropriate conditions to generate fast growing friable calli with detectable levels of grindelic acid. Moreover, the same basal media supplemented with 20.0 µM IBA/4.4 µM BA was found to be optimal for cell growth in submerged cultures (µmax = 0.26 days-1) while the addition of 20.0 µM IBA/18.0 µM BA resulted in a relative higher metabolite production (4.55 mg/gDW) when the inocula was 5% (v/v). Furthermore, three different stress... Tipo: Journal article Palavras-chave: Cu2+; Dimethylsulfoxide permeabilization; Grindelane diterpenes; Jasmonic acid. Ano: 2005 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582005000300007 Registros recuperados: 1 Primeira ... 1 ... Última

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