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| Ndungu, K; Gitonga, P; Mulinge, M; Kangethe, J; Kibugu, J. K; Munga, L. K; Maina, N; Kagira, J. M; Ngae, G. N; Murilla, G. |
| The efficacy of Triladyl®, a commercial cryomedium for bull semen, in the cryopreservation of both human and animal infective trypanosomes as compared to EDTA Saline Glucose (ESG) 10% glycerol was evaluated in the current study. Cryopreserved Trypanosoma brucei rhodesiense, T. evansi, T. b. brucei and T. congolense were first propagated in irradiated mice. At the peak of parasitemia, parasites were harvested by cardiac puncture and 106,105,104103,102 and 10 dilutions made using whole blood bled from clean mice. These dilutions were divided into two equal portions of 0.5 ml each and cryopreserved in both ESG 10% glycerol and neat Triladly®. The procedure was also repeated with T. congolense and T. vivax species of trypanosomes directly isolated from... |
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Palavras-chave: Trypanosoma; Cryopreservation. |
| Ano: 2009 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/2500 |
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| Ndungu, K; Kagira, J.M; Ngotho, J.M; Ouma, J; Bett, B; Gitonga, P. |
| Clinical and pathological characterisation of blood stream (BSF) and cerebrospinal fluid (CSF) forms of Trypanosoma brucei rhodesiense trypanosome isolated from a sleeping sickness patient were investigated in rabbits. The study aimed at investigating whether there is any significant difference in clinical and pathological presentation in rabbits infected by the two forms of trypanosomes. Each form of parasite was inoculated into five rabbits at 104 trypanosomes/ml while five rabbits were used as un-infected controls. Parasitaemia development, body temperature, packed cell volume (PCV), body weight, food and water intake, heartbeat and respiration were monitored daily for 30 days post infection when the experiment was terminated. Pathological changes were... |
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Palavras-chave: Pathogenicity; Rabbits; T. b. rhodesiense; CSF and Blood stream forms. |
| Ano: 2009 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/2387 |
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