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| Arcos-Ortega,Guadalupe Fabiola; Chan-Kuuk,Rafael Antonio; González-Kantún,Wilma Aracely; Souza-Perera,Ramón; Nakazawa-Ueji,Yumi Elena; Avilés-Berzunza,Elidé; Godoy-Hernández,Gregorio; Lawton,Michael A; Zúñiga Aguilar,José Juan. |
| Most of the pepper species of the genus Capsicum have been recalcitrant to efficient Agrobacterium tumefaciens-mediated stable or transient, genetic transformation. In the present work, we optimized a protocol for transient transformation of the Habanero pepper (Capsicum chinense Jacq.) through the standardization of several experimental factors. These included the age of the plants, the temperature, the length of co-cultivation, the application of a negative (vacuum) and/or a positive (infiltration) pressure, along with micro injection, the use of acetosyringone during the bacterial culturing, and modification of the pH during the GUS assay to eliminate the endogenous β-glucuronidase activity. The standardized protocol, which yielded nearly 55%... |
| Tipo: Journal article |
Palavras-chave: Cocultivation; GUS assay. |
| Ano: 2010 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582010000400007 |
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| Solís-Ramos,Laura Yesenia; González-Estrada,Tomas; Andrade-Torres,Antonio; Godoy-Hernández,Gregorio; Castaño de la Serna,Enrique. |
| The gene uidA, codes for β-glucuronidase, which is one of the reporters more frequently utilized in transgenic plants. However, this can only be use if the selected organism does not present endogenous GUS-like activity. In tissues of C. chinense we found a GUS-like activity showing different levels of intensity. Histochemical screening showed that endogenous GUS-like activity decreased, or reduced significantly, in almost all tissues with exception of stament, when phosphate buffer was adjusted to pH 8. Subsequently, C. chinense zygotic embryo explants were transient transformed with Agrobacterium tumefaciens LBA4404 (pCAMBIA2301) and plantlets regenerated were histochemically stained in phosphate buffer pH 8. Observations of incubated tissues of... |
| Tipo: Journal article |
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| Ano: 2010 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582010000400002 |
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