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Elicitation of peroxidase activity in genetically transformed root cultures of Beta vulgaris L. Electron. J. Biotechnol.
Rudrappa,Thimmaraju; Neelwarne,Bhagyalakshmi; Lakshmanan,Venkatachalam; Reddampalli Venkataramareddy,Sreedhar; Gokare Aswathanarayana,Ravishankar.
Genetically transformed roots of red beet produce copious levels of peroxidase (POD) - a multifunctional enzyme with a number of commercial applications. In an effort to elicit the POD activity, the cultures were treated with biotic elicitors such as dry cell powders of microbial cultures (0.1-0.5% w/v) and the respective culture filtrates (1-5% v/v). Similarly, abiotic elicitors, particularly metal ions (2-8 folds of that present in the nutrient medium), the plant hormone Thidiazuron (at 0.25-1 ppm) and other bio-molecules such as Glutathione (at 0.5-10 mM) and Methyl jasmonate (at 20-100 µM) were used. It was observed that dry cell powder of Candida versatilis significantly elicited the enzyme activity (3.52-fold higher than the control) followed by...
Tipo: Journal article Palavras-chave: Aspergillus; Calcium; Culture filtrate; Dried cell powder; Elicitor; Glutathione; Methyljasmonate; Rhizophus; Thidiazuron.
Ano: 2006 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582006000500006
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