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RNA-binding proteins and their role in the regulation of gene expression in Trypanosoma cruzi and Saccharomyces cerevisiae Genet. Mol. Biol.
Oliveira,Camila; Faoro,Helisson; Alves,Lysangela Ronalte; Goldenberg,Samuel.
Abstract RNA-binding proteins (RBPs) have important functions in the regulation of gene expression. RBPs play key roles in post-transcriptional processes in all eukaryotes, such as splicing regulation, mRNA transport and modulation of mRNA translation and decay. RBPs assemble into different mRNA-protein complexes, which form messenger ribonucleoprotein complexes (mRNPs). Gene expression regulation in trypanosomatids occurs mainly at the post-transcriptional level and RBPs play a key role in all processes. However, the functional characterization of RBPs in Trypanosoma cruzi has been impaired due to the lack of reliable reverse genetic manipulation tools. The comparison of RBPs from Saccharomyces cerevisiae and T. cruzi might allow inferring on the function...
Tipo: Info:eu-repo/semantics/article Palavras-chave: RNA-binding proteins; Trypanosoma cruzi; Saccharomyces cerevisiae; Gene expression regulation.
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572017000100022
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Darcy Fontoura de Almeida (1930-2014) Genet. Mol. Biol.
Vasconcelos,Ana Tereza Ribeiro de; Costa,Sergio Olavo Pinto da; Goldenberg,Samuel; Ferreira,Luís Carlos de Souza.
Tipo: Info:eu-repo/semantics/article
Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572014000300002
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Sera of Chagasic patients react with antigens from the tomato parasite Phytomonas serpens Biol. Res.
Graça-de Souza,Viviane K; Monteiro-Góes,Viviane; Manque,Patrício; Souza,Tatiana A.C.B; Corrêa,Paulo R.C.; Buck,Gregory A; Ávila,Andréa R; Yamauchi,Lucy M; Pinge-Filho,Phileno; Goldenberg,Samuel; Krieger,Marco A; Yamada-Ogatta,Sueli F.
The genus Phytomonas comprises trypanosomatids that can parasitize a broad range of plant species. These fagellates can cause diseases in some plant families with a wide geographic distribution, which can result in great economic losses. We have demonstrated previously that Phytomonas serpens 15T, a tomato trypanosomatid, shares antigens with Trypanosoma cruzi, the agent of human Chagas disease. Herein, two-dimensional gel electrophoresis (2-DE) and mass spectrometry (MS) were used to identify proteins of P. serpens 15T that are recognized by sera from patients with Chagas disease. After 2D-electrophoresis of whole-cell lysates, 31 peptides were selected and analyzed by tandem mass spectrometry. Twenty-eight polypeptides were identifed, resulting in 22...
Tipo: Journal article Palavras-chave: Phytomonas serpens; Trypanosoma cruzi; Antigenic cross-reactivity; Trypanosomatids.
Ano: 2010 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602010000200011
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