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	Provedor de dados BJMBR (1) Genet. Mol. Biol. (1) Autor Goldman,G.H. (2) Goldman,M.H.S. (2) Molfetta,J.B. (2) Angelo,P.C.S. (1) Brito,M.S. (1) Ferraz,M.T. (1) Figueiredo,L.H.M. (1) Figueiredo,R.C. (1) Quiapin,A.C. (1) Santos,R.V. (1) Silva,I. da (1) Silva,L.L.P. da (1) Silva,L.R. (1) Vitorelli,P.M. (1) Mais... Palavra-chave Glycine-rich protein (1) Nicotiana tabacum (1) Pistil expression (1) RNA-binding protein (1) Stress conditions (1) Virus infection (1) Mais... Tipo do documento Info:eu-repo/semantics/article (2) Ano 2002 (1) 2001 (1) País Brazil (2) Idioma Inglês (2)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 2 Primeira ... 1 ... Última A tobacco cDNA reveals two different transcription patterns in vegetative and reproductive organs BJMBR Silva,I. da; Angelo,P.C.S.; Molfetta,J.B.; Ferraz,M.T.; Silva,L.L.P. da; Goldman,G.H.; Goldman,M.H.S.. In order to identify genes expressed in the pistil that may have a role in the reproduction process, we have established an expressed sequence tags project to randomly sequence clones from a Nicotiana tabacum stigma/style cDNA library. A cDNA clone (MTL-8) showing high sequence similarity to genes encoding glycine-rich RNA-binding proteins was chosen for further characterization. Based on the extensive identity of MTL-8 to the RGP-1a sequence of N. sylvestris, a primer was defined to extend the 5' sequence of MTL-8 by RT-PCR from stigma/style RNAs. The amplification product was sequenced and it was confirmed that MTL-8 corresponds to an mRNA encoding a glycine-rich RNA-binding protein. Two transcripts of different sizes and expression patterns were... Tipo: Info:eu-repo/semantics/article Palavras-chave: Glycine-rich protein; Nicotiana tabacum; Pistil expression; RNA-binding protein; Stress conditions; Virus infection. Ano: 2002 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2002000800001 Dissecting the sugarcane expressed sequence tag (SUCEST) database: unraveling flower-specific genes Genet. Mol. Biol. Figueiredo,R.C.; Brito,M.S.; Figueiredo,L.H.M.; Quiapin,A.C.; Vitorelli,P.M.; Silva,L.R.; Santos,R.V.; Molfetta,J.B.; Goldman,G.H.; Goldman,M.H.S.. There are almost 260,000 independent clones sequenced from the 5’ end in the Sugarcane Expressed Sequence Tag (SUCEST) database, which have been obtained from 37 cDNA libraries prepared from different tissues. This large number of expressed sequence tags (ESTs) provides an opportunity, unprecedented in plants, to perform ‘digital differential screening’ on selected cDNA libraries. In general, the frequency of a particular EST correlates with transcript accumulation in the tissues from which the cDNA libraries were constructed, so it is possible to compare the whole transcriptome from different tissues using computer-assisted analysis of an EST database. In our research we analyzed sugarcane ESTs according to tissue expression and identified more than 1,000... Tipo: Info:eu-repo/semantics/article Ano: 2001 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572001000100012 Registros recuperados: 2 Primeira ... 1 ... Última

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