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| Ximenes,A.A; Oliveira,G.A; Bittencourt-Cunha,P; Tomokyo,M; Leite,D.B; Folly,E; Golodne,D.M; Atella,G.C. |
| Lipid transport in arthropods is achieved by highly specialized lipoproteins, which resemble those described in vertebrate blood. Here we describe purification and characterization of the lipid-apolipoprotein complex, lipophorin (Lp), from adults and larvae of the cowpea weevil Callosobruchus maculatus. We also describe the Lp-mediated lipid transfer to developing oocytes. Lps were isolated from homogenates of C. maculatus larvae and adults by potassio bromide gradient and characterized with respect to physicochemical properties and lipid content. The weevil Lp (465 kDa) and larval Lp (585 kDa), with hydrated densities of 1.22 and 1.14 g/mL, contained 34 and 56% lipids and 9 and 7% carbohydrates, respectively. In both Lps, mannose was the predominant... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Callosobruchus maculatus; Lipophorin; Lipid transport; Oogenesis; Hydrocarbons. |
| Ano: 2008 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2008000100004 |
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