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| Soares,Elisângela França; Silva,Anna Carolina da; Queiroz,Alana Emilia Soares de França; Gomes,José Erick Galindo; Herculano,Polyanna Nunes; Moreira,Keila Aparecida. |
| Breadfruit is an exotic tree in Brazil, very well acclimatized. Despite of being a laticifer plant, the knowledge about its latex is scarce. However, it is known that proteolytic enzymes represents over 50% of the latex composition in laticifers plants. The aim of this study was to evaluate the potential of breadfruit (Artocarpus altilis var. Apyrena) latex as a source of milk clotting proteases and partially characterize it. The proteolytic activity of the fraction of crude extract was assessed using azocasein and quantitation of total protein, the bicinchoninic acid (BCA). Milk-clotting activity was analyzed using skim milk at 12%. The enzyme activity was analyzed under different temperature conditions (35 to 80°C) and pH (5.8 to 10.7) presenting optimum... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Enzimas coagulantes do leite; Atividade proteolítica; Coagulante vegetal; Fruta-pão. |
| Ano: 2015 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782015000100149 |
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