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| Moreira,Mônica; Adamoski,Douglas; Sun,Jiufeng; Najafzadeh,Mohammad Javad; Nascimento,Mariana Machado Fidelis do; Gomes,Renata Rodrigues; Barbieri,Dicler de Sant'Anna; Glienke,Chirlei; Klisiowicz,Débora do Rocio; Vicente,Vânia Aparecida. |
| The aim of this study was to develop and evaluate a padlock probe based on the Rolling Circle Amplification (RCA), which targeted to 16S-23S rDNA region of S. mutans. The specificity of developed padlock probe was tested for DNA within a panel strains, including S. mutans isolated from the saliva and reference strains of the genus Streptococcus, as well as total DNA samples of biofilm and saliva. The results were positive either for DNA samples of S. mutans or DNA samples recovered from the biofilm and saliva revealing the specificity of designed padlock probe. The padlock probe based on the RCA was proved to be an effective, reproducible method for S. mutansdetection and demonstrated the possibility of a rapid detection and accurate identification of S.... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Padlock probe; RCA; Streptococcus mutans. |
| Ano: 2015 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132015000100054 |
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| Fornari,Gheniffer; Vicente,Vania Aparecida; Gomes,Renata Rodrigues; Muro,Marisol Dominguez; Pinheiro,Rosangela Lameira; Ferrari,Carolina; Herkert,Patricia Fernanda; Takimura,Marcos; Carvalho,Newton Sérgio de; Queiroz-Telles,Flavio. |
| Abstract Vulvovaginal candidiasis affects women of reproductive age, which represents approximately 15–25% of vaginitis cases. The present study aimed to isolate and characterize yeast from the patients irrespective of the presentation of clinical symptoms. The isolates were subjected to in vitro susceptibility profile and characterization by molecular markers, which intended to assess the distribution of species. A total of 40 isolates were obtained and identified through the CHROMagar, API20aux and by ITS and D1/D2 regions sequencing of DNAr gene. Candida albicans strains were genotyped by the ABC system and the isolates were divided into two genotypic groups. The identity of the C. albicans, C. glabrata, C. guilliermondii, C. kefyr and Saccharomyces... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Vulvovaginal candidiasis; In vitro susceptibility; Variability genetic. |
| Ano: 2016 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822016000200373 |
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