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Production of β-glucosidase on solid-state fermentation by Lichtheimia ramosa in agroindustrial residues: Characterization and catalytic properties of the enzymatic extract Electron. J. Biotechnol.
Garcia,Nayara Fernanda Lisboa; Santos,Flávia Regina da Silva; Gonçalves,Fabiano Avelino; Paz,Marcelo Fossa da; Fonseca,Gustavo Graciano; Leite,Rodrigo Simões Ribeiro.
Background β-Glucosidases catalyze the hydrolysis of cellobiose and cellodextrins, releasing glucose as the main product. This enzyme is used in the food, pharmaceutical, and biofuel industries. The aim of this work is to improve the β-glucosidase production by the fungus Lichtheimia ramosa by solid-state fermentation (SSF) using various agroindustrial residues and to evaluate the catalytic properties of this enzyme. Results A high production of β-glucosidase, about 274 U/g of dry substrate (or 27.4 U/mL), was obtained by cultivating the fungus on wheat bran with 65% of initial substrate moisture, at 96 h of incubation at 35°C. The enzymatic extract also exhibited carboxymethylcellulase (CMCase), xylanase, and...
Tipo: Journal article Palavras-chave: Cellobiase; Cellulases and hemicellulases; Industrial enzymes; Microbial enzymes.
Ano: 2015 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582015000400010
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