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	Provedor de dados BJMBR (2) Autor Gontijo,C.M. (2) Andrade,S.P. (1) Cara,D.C. (1) Lima,S.L. (1) Machado,C.B. (1) Oliveira,F.M. (1) Pereira,M.A.C. (1) Silva,C.A. (1) Silva-Neto,A.F. (1) Velarde,D.T. (1) Mais... Palavra-chave Antigen absorption (1) Gut mucosa (1) Immunization (1) Implant (1) Lipid-protein conjugates (1) Oral tolerance (1) Ovalbumin (1) Sponge (1) Vaccine (1) Mais... Tipo do documento Info:eu-repo/semantics/other (2) Ano 1999 (1) 1998 (1) País Brazil (2) Idioma Inglês (2)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 2 Primeira ... 1 ... Última Coupling of palmitate to ovalbumin inhibits the induction of oral tolerance BJMBR Oliveira,F.M.; Silva-Neto,A.F.; Silva,C.A.; Gontijo,C.M.. Oral tolerance is a phenomenon that may occur in animals exposed to protein antigens for the first time by the oral route. They become unable to produce immune responses at the levels normally observed when they are immunized parenterally with antigen in the presence of adjuvants. Lipids have been used as adjuvants for both parenteral and oral immunization. In the present study we coupled ovalbumin with palmitate residues by incubating the protein with the N-hydroxysuccinimide palmitate ester and tested the preparation for its ability to induce oral tolerance. This was performed by giving 20 mg of antigen to mice by the oral route 7 days prior to parenteral immunization in the presence of Al(OH)3. Mice were bled one week after receiving a booster that was... Tipo: Info:eu-repo/semantics/other Palavras-chave: Oral tolerance; Lipid-protein conjugates; Antigen absorption; Gut mucosa; Ovalbumin. Ano: 1998 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998001100009 Immunization by subcutaneous implants of polyester-polyurethane sponges coupled with antigen BJMBR Lima,S.L.; Machado,C.B.; Pereira,M.A.C.; Cara,D.C.; Velarde,D.T.; Andrade,S.P.; Gontijo,C.M.. A new protocol is described for immunization of outbred Swiss mice. The procedure is based on subcutaneous implantation of antigen-coupled polyester-polyurethane sponges cut into disks of 10 mm in diameter vs 2 mm in thickness. Antigen coupling was performed by overnight incubation of the sponge with a solution of ovalbumin (Ova) (2 mg/ml) diluted in sodium carbonate buffer, pH 9.6. The amount of ovalbumin that was taken up by the sponge was between 71.4 to 82.5 µg. This was estimated by comparing the Ova absorbance at 280 nm in coating buffer solutions before and after incubation. To compare the efficiency of the proposed method, experimental groups immunized with the antigen in the presence of adjuvants (10 µg in Al(OH)3 or 100 µg in complete Freund's... Tipo: Info:eu-repo/semantics/other Palavras-chave: Sponge; Implant; Immunization; Vaccine. Ano: 1999 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999000400011 Registros recuperados: 2 Primeira ... 1 ... Última

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