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	Provedor de dados BABT (2) Autor Goyal,Arun (2) Das,Debasish (1) Kothari,Damini (1) Patel,Seema (1) Shukla,Rishikesh (1) Shukla,Shraddha (1) Mais... Palavra-chave Central Composite Design (1) Dextran (1) Glucan (1) Glucansucrase (1) Pediococcus pentosaceus (1) Plackett-Burman Design (1) Response Surface Methodology (1) Tween 80 (1) Weissella confusa (1) Mais... Tipo do documento Info:eu-repo/semantics/article (2) Ano 2011 (2) País Brazil (2) Idioma Inglês (2)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 2 Primeira ... 1 ... Última Optimization of Fermentation Medium for Enhanced Glucansucrase and Glucan Production from Weissella confusa BABT Shukla,Shraddha; Goyal,Arun. A glucan producing Weissella confusa isolated from fermented cabbage, as identified earlier, was used for optimization of its fermentation medium. The effects of various macronutrients such as sucrose, glucose as cosubstrate, yeast extract, beef extract, peptone, sodium acetate, K2HPO4 and Tween 80 were studied on glucansucrase and glucan production from Weissella confusa. The medium used as control gave 6.0 U/ml enzyme activity and 34 mg/ml glucan concentration. Sucrose (5%), glucose as cosubstrate (5% for glucansucrase and 3% for glucan production), Tween 80 (0.1%), yeast extract (1.5%), Peptone (2.0%) and K2HPO4 (1.5%) were effective nutrients displaying higher glucansucrase and glucan production giving 18.2, 18.0, 7.0, 6.4, 6.2 and 6.4 U/ml enzyme... Tipo: Info:eu-repo/semantics/article Palavras-chave: Weissella confusa; Glucansucrase; Glucan; Tween 80. Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132011000600006 Scale up of dextran production from a mutant of Pediococcus pentosaceus (SPAm) using optimized medium in a bioreactor BABT Patel,Seema; Kothari,Damini; Shukla,Rishikesh; Das,Debasish; Goyal,Arun. The mutant of Pediococcus pentosaceus (SPAm) produced earlier by UV-mutagenesis exhibiting higher dextransucrase activity as compared to wild-type was used. The generated mutant SPAm gave 12.2 mg/ml, a 20% higher dextran than wild-type. Response surface methodology was carried out for further enhancement of dextran production. To enhance dextran production by the mutant SPAm, Plackett-Burman Design and a 2² full factorial Central Composite Design was employed. After response optimization, the optimum concentration of sucrose and yeast extract was 5.115% (w/v) and 0.635% (w/v), respectively. The experimental values of dextran 36.0 mg/ml at flask level and 35.0 mg/ml at bioreactor level were in good agreement with the predicted value of 40.8 mg/ml. The... Tipo: Info:eu-repo/semantics/article Palavras-chave: Pediococcus pentosaceus; Dextran; Plackett-Burman Design; Central Composite Design; Response Surface Methodology. Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132011000600007 Registros recuperados: 2 Primeira ... 1 ... Última

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