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Occurrence and molecular analysis of quarantine virus in lily cultivation areas in Brazil PAB
Rivas,Eliana Borges; Bôdi,Estevão Cardoso de Almeida; Harakava,Ricardo; Gregori,Fabio; Gonçalves,Marcos Cesar.
Abstract: The objective of this work was to describe the occurrence of quarantine Tulip breaking virus (TBV, synonym Lily mottle virus - LMoV) and Lily symptomless virus (LSV), and their respective molecular analyses, to provide data for supporting TBV removal from the Brazilian A1 quarantine pest list, since this virus has spread among the main commercial lily crops in Brazil. The occurrence of these viruses was detected in 12 cultivation areas through multiplex reverse transcription (RT-PCR), using specific primers to genes encoding the respective coat proteins (CP). Eight fragments of 800 nucleotides (nt) obtained from the LMoV-infected lilies and nine fragments of 600 nt from LSV-infected lilies were sequenced. Phylogenetic tree reconstruction showed a...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Lilium; Lily mottle virus; Lily symptomless virus; Phylogeny; Recombination analysis; RT-PCR..
Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2016000500615
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