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Hidden heterochromatin: characterization in the Rodentia species Cricetus cricetus, Peromyscus eremicus (Cricetidae) and Praomys tullbergi (Muridae) Genet. Mol. Biol.
Paço,Ana; Adega,Filomena; Guedes-Pinto,Henrique; Chaves,Raquel.
The use of in situ restriction endonuclease (RE) (which cleaves DNA at specific sequences) digestion has proven to be a useful technique in improving the dissection of constitutive heterochromatin (CH), and in the understanding of the CH evolution in different genomes. In the present work we describe in detail the CH of the three Rodentia species, Cricetus cricetus, Peromyscus eremicus (family Cricetidae) and Praomys tullbergi (family Muridae) using a panel of seven REs followed by C-banding. Comparison of the amount, distribution and molecular nature of C-positive heterochromatin revealed molecular heterogeneity in the heterochromatin of the three species. The large number of subclasses of CH identified in Praomys tullbergi chromosomes indicated that the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Constitutive heterochromatin; In situ restriction endonuclease digestion; Rodentia.
Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572009000100009
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Chemical carcinogenesis Anais da ABC (AABC)
Oliveira,Paula A.; Colaço,Aura; Chaves,Raquel; Guedes-Pinto,Henrique; De-La-Cruz P.,Luis F.; Lopes,Carlos.
The use of chemical compounds benefits society in a number of ways. Pesticides, for instance, enable foodstuffs to be produced in sufficient quantities to satisfy the needs of millions of people, a condition that has led to an increase in levels of life expectancy. Yet, at times, these benefits are offset by certain disadvantages, notably the toxic side effects of the chemical compounds used. Exposure to these compounds can have varying effects, ranging from instant death to a gradual process of chemical carcinogenesis. There are three stages involved in chemical carcinogenesis. These are defined as initiation, promotion and progression. Each of these stages is characterised by morphological and biochemical modifications and result from genetic and/or...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Cancer stages; Carcinogenesis evaluation; Chemical carcinogens; Chemical carcinogenesis.
Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652007000400004
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