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| Marcelino,Francismar Corrêa; Guimarães,Marta Fonseca Martins; De-Barros,Everaldo Gonçalves. |
| The increasing presence of products derived from genetically modified (GM) plants in human and animal diets has led to the development of detection methods to distinguish biotechnology-derived foods from conventional ones. The conventional and real-time PCR have been used, respectively, to detect and quantify GM residues in highly processed foods. DNA extraction is a critical step during the analysis process. Some factors such as DNA degradation, matrix effects, and the presence of PCR inhibitors imply that a detection or quantification limit, established for a given method, is restricted to a matrix used during validation and cannot be projected to any other matrix outside the scope of the method. In Brazil, sausage samples were the main class of... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: PCR quantitative; GMO; Sausage; Transgenic residues. |
| Ano: 2008 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612008000500007 |
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| Carmo,Fausto Moreira da Silva; Guimarães,Simone Eliza Facioni; Lopes,Paulo Sávio; Pires,Aldrin Vieira; Guimarães,Marta Fonseca Martins; Silva,Marcos Vinícius Gualberto Barbosa da; Schierholt,Alex Sandro; Silva,Kleibe de Moraes e; Gomide,Lúcio Alberto de Miranda. |
| We studied the phenotypic effects of polymorphisms at the MYF5 gene in a divergent F2 swine population and found that one polymorphism was due to an insertion and another to a deletion. The genotypes of 359 F2 animals were obtained and the Normal/Normal (NN) and Normal/Insertion (NI) genotypes analyzed to determine associations with phenotypic data for performance, carcass and meat quality traits. Significant differences were observed (p < 0.05) between NN and NI animals for drip (NN = 3.14 ± 1.56; NI = 3.69 ± 2.78%), cooking (NN = 32.26 ± 2.41; NI = 33.21 ± 2.31%) and total loss (NN = 34.16 ± 2.63 and NI = 34.97 ± 2.08%). The Deletion marker was not statistically tested. The results indicate that the allelic variant Insertion is associated with a... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Myogenesis; DNA sequencing; Pig production; PCR. |
| Ano: 2005 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572005000300004 |
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| Nascimento,Carlos Souza do; Machado,Marco Antonio; Martinez,Mario Luiz; Silva,Marcos Vinícius G. Barbosa da; Guimarães,Marta Fonseca Martins; Campos,Ana Lúcia; Azevedo,Ana Luisa Sousa; Teodoro,Roberto Luiz; Verneque,Rui da Silva; Guimarães,Simone Eliza Facioni; Oliveira,Denise Aparecida Andrade. |
| The effect of the bovine major histocompatibility complex (BoLA) locus on animal health may be due to a direct action of its alleles on immune functions, whereas its indirect effect on production traits might be explained by the better general health conditions of more productive animals. In the present study, the BoLA-DRB3 gene was investigated in 1058 cows belonging to seven Brazilian Gyr Dairy herds (Bos indicus, Zebu cattle). A total of 37 alleles were identified, 15 of them described for the first time in a Zebu breed. A highly significant association (p < 0.02) was observed between allele *54 and a decrease (-26.1 kg) in milk protein yield and there was a significant association (p < 0.05) between this allele and lower (-26.07 kg) milk fat... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Cattle; Marker-assisted selection; Molecular markers; PCR-RFLP. |
| Ano: 2006 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572006000400011 |
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| Silva,Marcos Vinicius Gualberto Barbosa da; Martinez,Mário Luiz; Machado,Marco Antonio; Nascimento,Carlos Souza do; Campos,Ana Lúcia; Guimarães,Marta Fonseca Martins; Azevedo,Ana Luisa Sousa; Moita,Antônia Kécya França; Lui,Jeffrey Frederico. |
| O objetivo deste trabalho foi avaliar a associação dos polimorfismos dos genes bGH, IGF-1 e PIT-1 com características de peso e ganho de peso numa população F2 de bovinos (Gir x Holandês), pela técnica de PCR-RFLP. As freqüências alélicas A e B, do gene PIT-1, e dos genótipos AA, AB e BB, nas populações parentais foram semelhantes entre si, mas diferentes das freqüências nas populações cruzadas F1 e F2, para esse gene. Quanto ao gene bGH, os animais da raça Holandesa apresentaram freqüência de 100% para o alelo E e os animais da raça Gir, 92% para o alelo F, resultando em alta freqüência de indivíduos heterozigotos nas populações F1 e F2. Quanto ao gene IGF-1, todos os animais da raça Holandesa eram heterozigotos (AB) e, nos animais Gir, a maioria dos... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Marcadores moleculares; PCR-RFLP. |
| Ano: 2006 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2006000600013 |
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| Arcuri,Pedro Braga; Odenyo,Agnes Awino; Arcuri,Edna Froeder; Ribeiro,Marlice Teixeira; Guimarães,Marta Fonseca Martins; Carneiro,Jailton da Costa. |
| The objective of this work was to isolate and characterize tannin-tolerant ruminal bacteria from crossbred Holstein x Zebu cows fed a chopped mixture of elephant grass (Pennisetum purpureum), young stems of "angico-vermelho" (Parapiptadenia rigida), and banana tree (Musa sp.) leaves. A total of 117 bacteria strains were isolated from enrichment cultures of rumen microflora in medium containing tannin extracts. Of these, 11 isolates were able to tolerate up to 3 g L-1 of tannins. Classical characterization procedures indicated that different morphological and physiological groups were represented. Restriction fragments profiles using Alu1 and Taq1 of 1,450 bp PCR products from the 16S rRNA gene grouped the 11 isolates into types I to VI. Sequencing of 16S... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Butyrivibrio fibrisolvens; Streptococcus bovis; Anti-nutritional polyphenolics; Ruminant nutrition. |
| Ano: 2011 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2011000300007 |
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| Mello,Fernanda de; Guimarães,Marta Fonseca Martins; Cobuci,Jaime Araújo; Silva,Marcos Vinícius Gualberto Barbosa da; Braccini Neto,José; Paiva,Daisyléa de Souza. |
| Objetivou-se obter os índices de diversidade genética para o SNP (single nucleotide polymorphism) do íntron 4 do gene da osteopontina (OPN) para 434 animais (87 touros e 347 vacas) participantes do Teste de Progênie da raça girolando no Brasil. Para a amplificação, foram utilizados primers descritos para a raça holandesa, e a diferenciação dos alelos C/T desse SNP foi obtida por meio da técnica de PCR-RFLP. As frequências genotípicas TT (52,53%), CT (38,71%) e CC (8,76%) e as frequências alélicas de T (71,9%) e C (28,1%) indicam que a população encontra-se em Equilíbrio de Hardy-Weinberg (EHW). Apesar de o loco do gene OPN estar em EHW, a frequência superior do alelo T do SNP nesses animais pode sugerir uma tendência de fixação do alelo T na raça. Não foi... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Bovinos leiteiros; Polimorfismo; Produção de leite; SNP; SPP1. |
| Ano: 2011 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982011001100013 |
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| Miranda-Furtado,Cristiana Libardi; Silva,Priscila Vendramini; Guimarães,Marta Fonseca Martins; Serão,Nicola Vergara Lopes; Guimarães,José Domingos; Guimarães,Simone Eliza Facioni. |
| The main purpose of the present study was to identify for candidate genes related to ovulation in swines. To do so, it was investigated in ovarian follicular cells through quantitative real-time PCR the differential expression of the following genes: steroidogenic acute regulator (STAR), GATA-binding protein 4 (GATA), prostaglandin F2α (PGF2α), progesterone receptor (P4R), follicle-stimulating hormone receptor (FSHR), and cytochrome P450 aromatase (CYP19). These genes encode hormone receptors (FSHR and P4R), hormone (PGF2α), steroidogenic proteins (STAR and CYP19) and transcription factor (GATA). Folicular cells were collected from sows with high and low number of piglets/litters during the follicular phase of the estrus cycle. There was difference in... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Candidate gene; Litter size; Ovulation rate; Pig production; Quantitative real-time PCR. |
| Ano: 2010 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982010000500012 |
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