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| Carvalho,Carlos Henrique S.; Zehr,Usha B.; Gunaratna,Nilupa; Anderson,Joseph; Kononowicz,Halina H.; Hodges,Thomas K.; Axtell,John D.. |
| The results presented in this work support the hypothesis that Agrobacterium-mediated transformation of sorghum is feasible, analogous to what has been demonstrated for other cereals such as rice, maize, barley and wheat. The four factors that we found most influenced transformation were: the sensitivity of immature sorghum embryos to Agrobacterium infection, the growth conditions of the donor plant, type of explant and co-cultivation medium. A major problem during the development of our protocol was a necrotic response which developed in explants after co-cultivation. Immature sorghum embryos proved to be very sensitive to Agrobacterium infection and we found that the level of embryo death after co-cultivation was the limiting step in improving... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Agrobacterium tumefaciens; GUS; Transformation; Sorghum. |
| Ano: 2004 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572004000200022 |
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