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Degradation of 2,4,6-trinitrotoluene by P. aeruginosa and characterization of some metabolites BJM
Mercimek,Hatice Aysun; Dincer,Sadik; Guzeldag,Gulcihan; Ozsavli,Aysenur; Matyar,Fatih; Arkut,Afet; Kayis,Fikret; Ozdenefe,Melis Sumengen.
Degradation of 2,4,6-trinitrotoluene (TNT), a nitroaromatic explosive found in the soil and ground water, was investigated using Pseudomonas aeruginosa in in vitroexperiments. Biodegradable abilitiy of this bacteria was performed with 50 and 75 mg L−1 TNT concentrations in a defined liquid medium for 96 h time period. Treatment of TNT in supernatant samples taken at 0, 6, 12, 24, 48, 72 and 96 h from agitated vessels was followed by reverse-phase high-performance liquid chromatography (HPLC). In cultures supplemented with 50 and 75 mgL−1 TNT, after 96 h of incubation 46% and 59% reduction were detected respectively. Two metabolites as degradation intermediates with nitrite release into the medium, 2,4-dinitrotoluene (2,4-DNT) and 4-aminodinitrotoluene...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Pseudomonas sp; Biotransformation; Nitroaromatic explosives; TNT.
Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822015000100103
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