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| Anibal,Paula Cristina; Sardi,Janaina de Cássia Orlandi; Peixoto,Iza Teixeira Alves; Moraes,Julianna Joanna de Carvalho; Höfling,José Francisco. |
| Candida-associated denture stomatitis is the most common form of oral candidal infection, with Candida albicans being the principal etiological agent. Candida adheres directly or via an intermediary layer of plaque-forming bacteria to denture acrylic. Despite antifungal therapy to treat denture stomatitis, infection is reestablished soon after the treatment ceases. In addition, many predisposing factors have been identified as important in the development of oral candidiasis, including malnourishment, common endocrine disorders, such as diabetis mellitus, antibacterial drug therapy, corticosteroids, radiotherapy and other immunocompromised conditions, such as acquired immunodeficiency syndrome (AIDS). These often results in increased tolerance to the most... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Candida spp.; Antifungals; Medicinal plants; Extracts; Essential oil. |
| Ano: 2010 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822010000400001 |
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| Rosa,Rosimeire Takaki; Napimoga,Marcelo Henrique; Höfling,José Francisco; Gonçalves,Reginaldo Bruno; Rosa,Edvaldo Antonio Ribeiro. |
| Twenty-one Streptococcus mutans strains were clustered by Multilocus Enzyme Electrophoresis (MLEE). Six isoenzymes showed strong infra-specific discriminatory power (M1P, MPI, PLP, NSP, GOT, and LAP). MLEE is a robust technique that may be used to explore clonal diversity of S. mutans isolates in epidemiological surveys. |
| Tipo: Info:eu-repo/semantics/other |
Palavras-chave: Streptococcus mutans; Isoenzymes; Clonal variability. |
| Ano: 2006 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822006000100003 |
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| Spolidorio,Denise Madalena Palomari; Höfling,José Francisco; Pizzolitto,Antônio Carlos; Rosa,Edvaldo Antonio; Negrini,Thaís de Cássia; Spolidorio,Luís Carlos. |
| The aim of this study was to determine whether random amplified polymorphic DNA (AP-PCR) analysis is able to differentiate genetically different clones of mutans streptococci, in 22 Brazilian family members. Stimulated saliva samples were collected from fathers, mothers and infants. For 5-18 months babies with erupting primary dentition, plaque samples were collected using sterile tooth pick tips. From these samples, mutans streptococci were isolated on SB-20 agar plates. After growth, representative colonies were identified by biochemical methods on the basis of carbohydrate fermentation. Streptococcus mutans isolates were obtained from all family members and AP-PCR typed separately with a random primer (OPA-13). Bacterial cell lysates were used as... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Streptococcus mutans; AP-PCR; Genetic polymorphism. |
| Ano: 2003 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822003000300006 |
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| Mardegan,Rita de Cássia; Klein,Marlise Inêz; Golvea,Magda Baglione; Rodrigues,Janaina Aparecida Oliveira; Gonçalves,Reginaldo Bruno; Höfling,José Francisco. |
| Candida albicans oral strains collected from caries-free and caries-active healthy children ranging from 24 to 36 months old, were studied. The aim of the study was to determine proteinase and phospholipase activities produced by Candida albicans in the two groups and to determine the phenotypic diversity of these enzymes based on genetic polymorphism using the AP-PCR method. Strains identified by morphological and fermentation tests as C. albicans were grown in proteinase and phospholipase agar media at 37ºC for 7 and 4 days, respectively. After the incubation period, the enzyme activity of the proteinase and phospholipase positive strains was measured. All strains were subjected to AP-PCR, using the arbitrary primer AP-3. The enzymatic analysis showed no... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Candida albicans; Genotyping; Proteinase; Phospholipase; Virulence. |
| Ano: 2006 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822006000100005 |
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| Boriollo,Marcelo Fabiano Gomes; Spolidorio,Denise Madalena Palomari; Barros,Letizia Monteiro; Bassi,Rodrigo Carlos; Garcia,José Antonio Dias; Costa,Ana Maria Duarte Dias; Rosa,Edvaldo Antonio Ribeiro; Höfling,José Francisco. |
| The genetic diversity of C. albicans oral isolates from 75 healthy schoolchildren from eight schools located in different geographic areas of Piracicaba city, São Paulo state, Brazil, was established using isoenzymes marker (Multilocus Enzyme Electrophoresis - MLEE) and cluster analysis. Patterns of monoclonal and polyclonal oral colonization by C. albicans within and between groups of schoolchildren were identified. However, significant divergence between the observed and the expected genotypic frequencies (Hardy-Weinberg equilibrium test) was not detected in the geographically adjacent groups, suggesting the hypothesis that populations of healthy schoolchildren do not correspond to the selection factor (differential survival) of strains. Two highly... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Candida albicans; Healthy schoolchildren; Geographic region; MLEE; Cluster analysis. |
| Ano: 2011 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822011000300023 |
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| Rodrigues,Cristina Crespo; Höfling,José Francisco; Boriollo,Marcelo Fabiano Gomes; Rodrigues,Janaina Aparecida de Oliveira; Azevedo,Ricardo Antunes de; Gonçalves,Reginaldo Bruno; Gomes,Luiz Humberto; Tavares,Flávio César Almeida. |
| The aim of the present research was to evaluate the protein polymorphism degrees among forty-eight C. albicans isolates from fourteen anatomical sites of clinical patients by polyacrylamide gel electrophoresis (SDS-PAGE) and numerical analyzes, in order to identify subspecies and their similarities in some infectious niches. Cell cultures were grown in YEPD medium, collected by centrifugation, and washed in cold saline solution. The whole-cell proteins were extracted by cell disruption using glass beads and submitted to SDS-PAGE technique. After electrophoresis, the protein bands were stained with coomassie-blue and analyzed by statistics package NTSYS-pc version 1.70 software. Similarity matrixes and dendrograms were generated by application of similarity... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Numerical analyzes; SDS-PAGE; C. albicans; Human anatomical sites. |
| Ano: 2004 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822004000100006 |
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| Anibal,Paula Cristina; Peixoto,Iza Teixeira Alves; Foglio,Mary Ann; Höfling,José Francisco. |
| Ethanolic crude extracts prepared from the arils and seeds, pericarp, peels and from the whole fruit of Punica granatum, known as pomegranate, had their antifungal activity tested against Candida spp. The ethanolic crude extracts were analyzed by Mass Spectrometry and yielded many compounds such as punicalagin and galladydilacton. The extracts from the pericarp and peel showed activity against Candida spp., with MICs of 125 µg/mL. The effect of pericarp and peel extracts upon the morphological and structure of C. albicans and C. krusei were examined by scanning and transmission electron microscopy, with the visualization of an irregular membrane and hyphae, formation of vacuoles and thickening of the cell wall. The data obtained revealed potential... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Punica granatum; Punicalagin; Antifungal activity; Candida spp.; Electron microscopy. |
| Ano: 2013 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822013000300027 |
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