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Trends in functional sugar research : Focus on prebiotic effects of functional sugar OAK
福島, 道広; 中村, 有美; 李, スルギ; 土平, 洋彰; 小林, 由佳; 川上, 秋桜; 岡田, 朋子; 島田, 謙一郎; 韓, 圭鎬; Fukushima, Michihiro; Nakamura, Yumi; Lee, Seulki; Tsuchihira, Hiroaki; Kobayashi, Yuka; Kawakami, Sakura; Okada, Tomoko; Shirmada, Ken-ichiro; Han, Kyu-Ho.
今回、我々は北海道十勝で生産された豆類やジャガイモ、新規作物として栽培開発を行っているチコリから難消化性糖質を分離、そのプレバイオティクス効果について検証した。 豆類では、澱粉の構造がレジスタントスターチタイプ2であり、この澱粉が加熱調理加工過程でタイプ3の老化澱粉とタンパク質の被膜が形成されることにより、消化性が低下する可能性が見い出された。その結果、ラットの盲腸内で酸発酵が増進し、短鎖脂肪酸の上昇とpHの低下が認められ、腸内環境を改善させることが明らかとなった。さらに、十勝の特産物であるジャガイモもエステル結合したリン酸基を持つ澱粉であり、レジスタントスターチタイプ2に分類される。この澱粉も加熱調理加工することによりタイプ3の老化澱粉が増加する可能性がある。このジャガイモ澱粉がラット盲腸内で短鎖脂肪酸量を増加させ、特に酪酸の総短鎖脂肪酸に対する比率の上昇は消化を刺激して、糞便重量を増加させることが明らかであり、酪酸の好材料であることが確認された。また、チコリ由来のイヌリンについても、十勝イヌリンがin vivoおよびin vitro研究で盲腸内微生物叢、特にLactobacillusおよびBifidobacteriumレベルを上昇させ、総短鎖脂肪酸濃度も増加させることが明らかとなり、従来の報告と一致していた。 以上の結果より、豆類およびジャガイモのデンプンにはレジスタントスターチの作用を有しており、また十勝で新規に栽培したチコリ由来のイヌリンにも腸内改善効果が認められ、機能性糖質としてプレバイオティクス作用を持つことが明らかとなった。
Ano: 2011 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/3321
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Some Bovine proteins behave as dietary fibres and reduce serum lipids in rats OAK
Ruvini, Liyanage; Hashimoto, Naoto; Han, Kyu-Ho; Kajiura, Teppei; Watanabe, Shoko; Shimada, Ken-ichiro; Sekikawa, Mitsuo; Ohba, Kiyoshi; Fukushima, Michihiro.
We examined the physiological importance of bovine dietary proteins in rats fed diets prepared from bovine Achilles’tendons and arteries. Rats were fed for 4 weeks, with 20% casein diet (CON), in comparison with two diets containing 15% casein and 5% of either bovine Achilles’ tendons (AC) or arteries (AR) protein preparations. The serum total cholesterol concentration and non-HDL cholesterol level in the AR fed group were significantly lower (P<0.05) than those in the CON fed group at the end of the 4 week feeding period. The hepatic mRNAs were measured, and the HMG-CoA reductase mRNA level was significantly lower (P<0.05) in AR fed group compared to CON fed group. Total hepatic cholesterol concentration, in rats fed AC was significantly...
Palavras-chave: Bovine Dietary proteins; Serum lipids; Dietary Fibres; Hepatic mRNAs.
Ano: 2007 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/745
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Porcine artery elastin preparation reduces serum cholesterol level in rats OAK
Liyanage, Ruvini; Nakamura, Yumi; Shimada, Ken-ichiro; Sekikawa, Mitsuo; Jayawardana, Barana Chaminda; Han, Kyu-Ho; Tomoko, Okada; Ohba, Kiyoshi; Takahata, Yoshihisa; Morimatsu, Fumiki; Fukushima, Michihiro; 福島, 道広; 島田, 謙一郎; 関川, 三男; 韓, 圭鎬.
The effect of porcine artery elastin on serum cholesterol level was investigated in rats fed a cholesterol-free diet. Rats were fed for 4 weeks, with a diet (ED) containing 15% casein and 5% of porcine artery elastin in comparison with a diet (CD) containing 20% casein. The total serum and non-HDL-cholesterol concentrations were lower (P<0.001) in ED-fed group than the CD-fed group at the end of the experiment. Caecal propionate concentration and Bifidobacterium and Lactobacillus population were higher (P<0.05) in ED-fed group than the CD-fed group. The results of this study suggest that porcine artery elastin could be considered as a functional dietary protein with hypocholesterolaemic ability. Favourable amino acid composition and lysine derived...
Ano: 2009 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/2681
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Relationships among alcoholic liver disease, antioxidants, and antioxidant enzymes OAK
Han, Kyu-Ho; Hashimoto, Naoto; Fukushima, Michihiro.
Excessive consumption of alcoholic beverages is a serious cause of liver disease worldwide. The meta-bolism of ethanol generates reactive oxygen species, which play a significant role in the deterioration of alcoholic liver disease (ALD). Antioxidant phytochemicals, such as polyphenols, regulate the expression of ALD-associated proteins and peptides, namely, catalase, superoxide dismutase, glutathione, glutathione peroxidase, and glutathione reductase. These plant antioxidants have electrophilic activity and may induce antioxidant enzymes via the Kelchlike ECH-associated protein 1-NF-E2-related factor-2 pathway and antioxidant responsive elements. Furthermore, these antioxidants are reported to alleviate cell injury caused by oxidants or inflammatory...
Palavras-chave: Electrophile; Mitogen-activating protein kinase; Plant antioxidants; Reactive oxygen species; Preconditioning.
Ano: 2016 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4425
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