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	Provedor de dados BJMBR (2) Autor Han,S.W. (2) Denapolis,P.M.A. (1) Kanashiro,C.A. (1) Krieger,J.E. (1) Merjan,A.J. (1) Moraes,J.Z. (1) Paiva,A.C.M. (1) Sacramento,C.B. (1) Mais... Palavra-chave Agonist-induced receptor internalization (1) Angiotensin II (1) Endocytosis (1) Fluorescence-tagged receptor (1) Hypoxia (1) Hypoxia-responsive element (1) Ischemic diseases (1) Normoxia (1) Nuclear angiotensin receptors (1) Renin-angiotensin system (1) SV40-DTS (1) Transfection efficiency (1) Mais... Tipo do documento Info:eu-repo/semantics/article (2) Ano 2010 (1) 2001 (1) País Brazil (2) Idioma Inglês (2)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 2 Primeira ... 1 ... Última Ligand-induced endocytosis and nuclear localization of angiotensin II receptors expressed in CHO cells BJMBR Merjan,A.J.; Kanashiro,C.A.; Krieger,J.E.; Han,S.W.; Paiva,A.C.M.. A construct (AT1R-NF) containing a "Flag" sequence added to the N-terminus of the rat AT1 receptor was stably expressed in Chinese hamster ovary cells and quantified in the cell membrane by confocal microscopy after reaction with a fluorescein-labeled anti-Flag monoclonal antibody. Angiotensin II bound to AT1R-NF and induced endocytosis with a half-time of 2 min. After 60-90 min, fluorescence accumulated around the cell nucleus, suggesting migration of the ligand-receptor complex to the nuclear membrane. Angiotensin antagonists also induced endocytosis, suggesting that a common step in the transduction signal mechanism occurring after ligand binding may be responsible for the ligand-receptor complex internalization. Tipo: Info:eu-repo/semantics/article Palavras-chave: Angiotensin II; Agonist-induced receptor internalization; Endocytosis; Fluorescence-tagged receptor; Nuclear angiotensin receptors; Renin-angiotensin system. Ano: 2001 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001000900011 Gene expression promoted by the SV40 DNA targeting sequence and the hypoxia-responsive element under normoxia and hypoxia BJMBR Sacramento,C.B.; Moraes,J.Z.; Denapolis,P.M.A.; Han,S.W.. The main objective of the present study was to find suitable DNA-targeting sequences (DTS) for the construction of plasmid vectors to be used to treat ischemic diseases. The well-known Simian virus 40 nuclear DTS (SV40-DTS) and hypoxia-responsive element (HRE) sequences were used to construct plasmid vectors to express the human vascular endothelial growth factor gene (hVEGF). The rate of plasmid nuclear transport and consequent gene expression under normoxia (20% O2) and hypoxia (less than 5% O2) were determined. Plasmids containing the SV40-DTS or HRE sequences were constructed and used to transfect the A293T cell line (a human embryonic kidney cell line) in vitro and mouse skeletal muscle cells in vivo. Plasmid transport to the nucleus was monitored by... Tipo: Info:eu-repo/semantics/article Palavras-chave: SV40-DTS; Hypoxia-responsive element; Transfection efficiency; Normoxia; Hypoxia; Ischemic diseases. Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2010000800005 Registros recuperados: 2 Primeira ... 1 ... Última

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