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| Sharmin,Shamima Akhtar; Alam,Md. Jahangir; Sheikh,Md. Mominul Islam; Sarker,Kanak Kanti; Khalekuzzaman,Muhammad; Haque,Md. Anwarul; Alam,Mohammad Firoz; Alam,Iftekhar. |
| In this work, plant regeneration via somatic embryogenesis was achieved from leaf and internode derived callus of Wedelia calendulacea, an endangered medicinal plant. Primary callus was induced by culturing leaf disc and internode explant on Murashige and Skoog medium supplemented with 2.0 mg L-1 of 2,4-D under light condition. Transfer of embryogenic callus on a reduced concentration of 2,4-D facilitated somatic embryo development while calluses remained unorganized at the same 2,4-D level. A histological analysis confirmed somatic embryo by revealing the presence of a closed vascular system in the developing embryos and lack of a vascularconnection with surrounding callus tissues. Somatic embryos germinated into plantlets upon transfer on MS medium... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: In vitro; Medicinal plant; Micropropagation; Somatic embryo; Threatened; Wedelia calendulacea. |
| Ano: 2014 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132014000300013 |
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