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| Dheilly, Alexandra; Soum-soutera, Emmanuelle; Klein, Geraldine; Bazire, Alexis; Compere, Chantal; Haras, Dominique; Dufour, Aurelie. |
| Biofilm formation results in medical threats or economic losses and is therefore a major concern in a variety of domains. In two-species biofilms of marine bacteria grown under dynamic conditions, Pseudoalteromonas sp. strain 3J6 formed mixed biofilms with Bacillus sp. strain 4J6 but was largely predominant over Paracoccus sp. strain 4M6 and Vibrio sp. strain D01. The supernatant of Pseudoalteromonas sp. 3J6 liquid culture (SN3J6) was devoid of antibacterial activity against free-living Paracoccus sp. 4M6 and Vibrio sp. D01 cells, but it impaired their ability to grow as single-species biofilms and led to higher percentages of nonviable cells in 48-h biofilms. Antibiofilm molecules of SN3J6 were able to coat the glass surfaces used to grow biofilms and... |
| Tipo: Text |
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| Ano: 2010 |
URL: http://archimer.ifremer.fr/doc/00004/11530/11317.pdf |
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| Paillard, Christine; Nicolas, Jean-louis; Le Chevalier, Patrick; Lambert, Christophe; Le Boulay, Christine; Berthe, Franck; Haras, Dominique. |
| The emergence of new vibriosis in the European Atlantic coasts has been associated with mass mortalities of molluscs causing important economic losses. For examples, Vibrio carchariae, a bacteria already isolated from shark, has been associated with a severe Haliotis epizooty in hatchery. In oysters, Crass()strea gigas, a strain closely related to Vibrio splendidus is suspected to be associated with summer mortalities. |
| Tipo: Text |
Palavras-chave: Pathogenic vibrios; Bivales; Vibriosis; Bacteriology. |
| Ano: 2001 |
URL: http://archimer.ifremer.fr/doc/2001/acte-3287.pdf |
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| Paillard, Christine; Gausson, Sabrina; Nicolas, Jean-louis; Le Pennec, Jean Paul; Haras, Dominique. |
| Vibrio tapetis is the marine bacterium responsible for the brown ring disease (BRD) affecting die manila clam, Ruditapes philippinarum. Identification of V tapelis has been previously performed using biochemical criteria and serological procedures. All of these methods are time consuming and ill-adapted to individual screening. This study describes an oligonucleotidic probe (Vt446) and two PCR primers, deduced from the 16S rDNA sequence, allowing a fast and specific V tapetis identification using dot blot hybridisation and species-specific primed PCR (SSP-PCR). The probe and primers have been tested on 60 strains, including referenced Vibrio sp., Gram negative and positive bacteria, marine bacteria samples and isolated clam bacteria. For all the 19 V... |
| Tipo: Text |
Palavras-chave: DOT BLOT hybridisation; SSP PCR; Vibriosis; Bivalve; Detection; Diagnostic. |
| Ano: 2006 |
URL: http://archimer.ifremer.fr/doc/2006/publication-1692.pdf |
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