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| Salin, Karine; Auer, Sonya K.; Villasevil, Eugenia M.; Anderson, Graeme J.; Cairns, Andrew G.; Mullen, William; Hartley, Richard C.; Metcalfe, Neil B.. |
| In recent years evolutionary ecologists have become increasingly interested in the effects of reactive oxygen species (ROS) on the life-histories of animals. ROS levels have mostly been inferred indirectly due to the limitations of estimating ROS from in vitro methods. However, measuring ROS (hydrogen peroxide, H2O2) content in vivo is now possible using the MitoB probe. Here, we extend and refine the MitoB method to make it suitable for ecological studies of oxidative stress using the brown trout Salmo trutta as model. The MitoB method allows an evaluation of H2O2 levels in living organisms over a timescale from hours to days. The method is flexible with regard to the duration of exposure and initial concentration of the MitoB probe, and there is no... |
| Tipo: Text |
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| Ano: 2017 |
URL: https://archimer.ifremer.fr/doc/00413/52494/53297.pdf |
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| Salin, Karine; Villasevil, Eugenia M.; Anderson, Graeme J.; Auer, Sonya K.; Selman, Colin; Hartley, Richard C.; Mullen, William; Chinopoulos, Christos; Metcalfe, Neil B.. |
| Many animals experience periods of food shortage in their natural environment. It has been hypothesised that the metabolic responses of animals to naturally‐occurring periods of food deprivation may have long‐term negative impacts on their subsequent life‐history. In particular, reductions in energy requirements in response to fasting may help preserve limited resources but potentially come at a cost of increased oxidative stress. However, little is known about this trade‐off since studies of energy metabolism are generally conducted separately from those of oxidative stress. Using a novel approach that combines measurements of mitochondrial function with in vivo levels of hydrogen peroxide (H2O2) in brown trout (Salmo trutta), we show here that fasting... |
| Tipo: Text |
Palavras-chave: High-resolution respirometry; In vivo; Liver atrophy; MitoB probe; Mitochondrial respiratory state. |
| Ano: 2018 |
URL: https://archimer.ifremer.fr/doc/00449/56022/57540.pdf |
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