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	Provedor de dados BJM (2) Autor Hartskeerl,Rudy (2) Anzai,Eleine Kuroki (1) Bourhy,Pascale (1) Dellagostin,Odir (1) Freitas,Julio Cesar de (1) Giraldi,Nilson (1) Kremer,Frederico (1) Lilenbaum,Walter (1) Ristow,Paula (1) Vasconcellos,Silvio (1) Vasconcellos,Silvio Arruda (1) Zacarias,Francielle Gibson da Silva (1) Mais... Palavra-chave Leptospira (2) Cattle (1) Culture (1) Diagnosis (1) Leptospirosis (1) PFGE (1) Rrs (1) Sequencing (1) Serogrouping (1) Mais... Tipo do documento Info:eu-repo/semantics/article (2) Ano 2014 (1) 2008 (1) País Brazil (2) Idioma Inglês (2)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 2 Primeira ... 1 ... Última Isolation of leptospira Serovars Canicola and Copenhageni from cattle urine in the state of Paraná, Brazil BJM Zacarias,Francielle Gibson da Silva; Vasconcellos,Silvio Arruda; Anzai,Eleine Kuroki; Giraldi,Nilson; Freitas,Julio Cesar de; Hartskeerl,Rudy. In 2001, 698 urine samples were randomly collected from cattle at a slaughterhouse in the State of Paraná, Brazil. Direct examination using dark field microscopy was carried out immediately after collection. Five putative positive samples were cultured in modified EMJH medium, yielding two positive cultures (LO-14 and LO-10). Typing with monoclonal antibodies revealed that the two isolates were similar to Canicola (LO-14) and Copenhageni (LO-10). Microscopic agglutination test results show that Hardjo is the most common serovar in cattle in Brazil. Rats and dogs are the common maintenance hosts of serovars Copenhageni and Canicola. The excretion of highly pathogenic serovars such as Copenhageni and Canicola by cattle can represent an increasing risk for... Tipo: Info:eu-repo/semantics/article Palavras-chave: Leptospira; Culture; Leptospirosis; Diagnosis; Cattle. Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822008000400028 Molecular characterization of the first leptospires isolated from goats in Brazil BJM Lilenbaum,Walter; Kremer,Frederico; Ristow,Paula; Dellagostin,Odir; Bourhy,Pascale; Hartskeerl,Rudy; Vasconcellos,Silvio. Two Leptospira sp. isolates were obtained by the first time from goats in Brazil and characterized by sequencing rrs, rpoB and secY genes, PFGE and typing with monoclonal antibodies. Both isolates are identical and belong to Leptospira santarosai. Analysis of the rrs and the rpoB genes sequences revealed 100% identity between the goat isolates and the Bananal reference strain. When secY sequences of the two isolates were compared to each other, it was observed that they had identical sequences. However, when compared to that of the Bananal reference strain, there were 15 mismatches along the 549 bp secY sequence. In conclusion, molecular methods are increasingly useful for the characterization of leptospires and allowed to identify those isolates of... Tipo: Info:eu-repo/semantics/article Palavras-chave: Leptospira; Serogrouping; Sequencing; PFGE; Rrs. Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000400050 Registros recuperados: 2 Primeira ... 1 ... Última

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