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| Registros recuperados: 18 | |
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| Hashimoto, Makoto; Nabeta, Kensuke; Murakami, Kentaro. |
| 3-Trifluoromethylphenyldiazirine based oleic acids derivatives are synthesized to elucidate the functions of specific activation of protein kinase C (PKC) with oleic acid. The synthetic route is based on the alkylation of phenolic derivative with oleic acid equivalent and the post-functionalization. of the compound to achieve radiolabeling. Several compounds have biological activity for PKC with similar efficacy with that of oleic acid. The results indicated that the diaizinyl oleic acid derivatives should be useful to study the specific functions of oleic acid for PKC. (C) 2003 Elsevier Science Ltd. All rights reserved. |
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Palavras-chave: PHENYLALANINE; DIAZIRINE; RESPONSES; ANALOGS; PROBES. |
| Ano: 2003 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/1324 |
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| Hashimoto, Makoto; Hatanaka, Yasumaru. |
| 3-Trifluoromethylphenyldiazirinylated diol derivative was utilized to introduce an aldehyde by periodate oxidation, followed by the formation of a Schiff base with biotin hydrazide on a PVDF membrane for post-labeling of photocrosslinked proteins. The biotin hydrazide was able to post-label the 2.5 x 10-13 mole of closslinked component for chemiluminescent visualization. |
| Ano: 2005 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/735 |
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| Hashimoto, Makoto; Hatanaka, Yasumaru; 橋本, 誠. |
| A novel photoreactive ceramide analogue, which contains (3-trifluoromethyl) phenyldiazirinyl lipid and biotinylated sphingosine, was synthesized. The probe was recognized as an antigen by anti-ceramide antibody and as a substrate for sphingolipid ceramide N-deacylase. These results indicate that the probe may be useful as a photoaffinity-biotinylating agent in sphingolipid studies. |
| Ano: 2008 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/2704 |
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| Hashimoto, Makoto; Hatanaka, Yasumaru; Nabeta, Kensuke. |
| A versatile synthesis of diazirine-based photoreactive fatty acid analogues is reported. The key step is phenoxy alkylation of diazirine with halo alkyl acid esters. The conditions described will be acceptable for the synthesis of various alkyl-length derivatives. The fatty acid derivatives are acceptors for reverse reactions of sphingolipid ceramide N-deacylase (SCDase), which catalyzes the condensation of psychosine and fatty acids to form photoreactive galactosylceramide. The photoreactive galactosylceramide can also be prepared with chemical synthesis, condensation of psychosine and fatty acid succinimidyl ester, and is recognized with anti-GarCer antibody both before and after irradiation. |
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Palavras-chave: DIAZIRINE. |
| Ano: 2002 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/736 |
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| Hashimoto, Makoto; Hatanaka, Yasumaru. |
| Post-biotinylation of the alkyl azide derivative of trifluoromethyl phenyldiazirine (TPD) was elucidated to apply a photoaffinity biotinylation technique. A photo-modified polyvinilidene difluoride (PVDF) membrane was used as a photolabeled component and we introduced biotin by Staudinger-Bertozzi ligation. The 15 pmol amount of biotinylated reagent was still effective for the visualization of cross-linked product on the matrix. The results show the potential utility of alkyl azide carrying TPD derivatives in the application of photoaffinity biotinylation, which could be useful for the ligands with tight structural requirements. |
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Palavras-chave: Diazirine; Photoaffinity label; Chemical biology; Staudinger reaction; Avidin-biotin. |
| Ano: 2005 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/832 |
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| Registros recuperados: 18 | |
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